Ye Zhi Ting, Tseng Shen Fu, Tsou Shang Xuan, Tsai Chun Wei
Department of Mechanical Engineering, Advanced Institute of Manufacturing with High-Tech Innovations, National Chung Cheng University, 168, University Rd., Min-Hsiung, Chia-Yi, 62102, Taiwan, ROC.
Graduate Institute of Photonics and Optoelectronics, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei, 106319, Taiwan, ROC.
Discov Nano. 2024 Jan 18;19(1):13. doi: 10.1186/s11671-024-03957-2.
Because the human eye cannot visually detect the results of direct bilirubin test papers accurately and quantitatively, this study proposes four different highly collimated mini light-emitting diodes (HC mini-LEDs) as light sources for detection. First, different concentrations of bilirubin were oxidized to biliverdin by FeCl on the test paper, and pictures were obtained with a smartphone. Next, the red, green, and blue (RGB) channels of the pictures were separated to average grayscale values, and their linear relationship with the direct bilirubin concentration was analyzed to detect bilirubin on the test paper noninvasively and quantitatively. The experimental results showed that when green HC mini-LEDs were used as the light sources and image analysis was performed using the G channel, for a direct bilirubin concentration range of 0.1-2 mg/dL, the G channel determination coefficient (R) reached 0.9523 and limit of detection was 0.459 mg/dL. The detection method proposed herein has advantages such as rapid analysis, noninvasive detection, and digitization according to RGB grayscale changes in the images of the detection test paper.
由于人眼无法准确、定量地视觉检测直接胆红素试纸的结果，本研究提出了四种不同的高准直微型发光二极管（HC微型发光二极管）作为检测光源。首先，在试纸上通过氯化铁将不同浓度的胆红素氧化为胆绿素，并用智能手机获取图片。接下来，将图片的红、绿、蓝（RGB）通道分离以得到平均灰度值，并分析它们与直接胆红素浓度的线性关系，以无创且定量地检测试纸上的胆红素。实验结果表明，当使用绿色HC微型发光二极管作为光源并使用G通道进行图像分析时，对于0.1 - 2mg/dL的直接胆红素浓度范围，G通道测定系数（R）达到0.9523，检测限为0.459mg/dL。本文提出的检测方法具有分析快速、无创检测以及根据检测试纸图像中的RGB灰度变化进行数字化等优点。
