School of Acupuncture-Moxibustion and Tuina, School of Health Preservation and Rehabilitation, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Department of Acupuncture and Moxibustion, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province.
Zhen Ci Yan Jiu. 2024 Jan 25;49(1):6-14. doi: 10.13702/j.1000-0607.20221405.
To observe the effect of electroacupuncture (EA) on changes of ventricular structure and function in rats with myocardial ischemia-reperfusion injury (MIRI), so as to explore its potential mechanisms underlying improvement of ventricular remodeling after MIRI.
Forty male SD rats were randomly divided into 4 groups：sham operation group, model group, EA group and medication (sacubactril valsartan, LCZ696) group, with 10 rats in each group. The MIRI model was established by ligation of the left anterior descending coronary artery and reperfusion. EA (2 Hz/100 Hz, 2 mA) was applied to bilateral "Neiguan" (PC6) for 20 min, once every other day for 21 d. Rats of the medication group received gavage of LCZ696 (60 mg·kg·d). After the intervention, echocardiography was used to detect the ejection fraction (EF) and fractional shortening (FS) of the left ventricle, and the contents of serum tumor necrosis factor-α(TNF-α), vascular cell adhesion molecule-1(VCAM-1) and intercellular cell adhesion molecule-1(ICAM-1) were assayed by enzyme-linked immunosorbent assay. The pathological changes of myocardial tissue were observed after HE staining. The Masson staining was used to evaluate the myocardial collagen deposition and myocardial fibrosis. The mRNA expression levels of collagen Ⅰ and Ⅲ and connective tissue growth factor (CTGF) in the myocardial tissue were detected by quantitative real-time PCR, and the expression levels of IL-1β and IL-18 were detected by Western blot.
In contrast to the sham operation group, the EF and FS levels of the left ventricle were ob-viously decreased (<0.001), while the contents of serum TNF-α, VCAM-1 and ICAM-1, the proportion of myocardial fibrosis area, the mRNA expression levels of myocardial collagen Ⅰ, collagen Ⅲ and CTGF, the expression levels of IL-1β and IL-18 were significantly increased (<0.001, <0.000 1, <0.05, <0.01) in the model group. Compared with the model group, the EF and FS levels were remarkably increased (<0.01), whereas the contents of serum TNF-α, VCAM-1 and ICAM-1, the proportion of myocardial fibrosis area, the mRNA expression levels of myocardial collagen Ⅰ, collagen Ⅲ and CTGF, and the expression levels of IL-1β and IL-18 were significantly down-regulated (<0.001, <0.01, <0.05) in both the medication and EA groups. No significant differences were found between the EA and medication groups in all the indexes mentioned above.
EA can improve the left-ventricular fibrosis and function, delay or reverse ventricular remodeling in MIRI rats, which may be related to its functions in down-regulating myocardial inflammatory response and mRNA expression levels of myocardial collagen Ⅰ, collagen Ⅲ and CTGF.
观察电针对心肌缺血再灌注损伤（MIRI）大鼠心室结构和功能变化的影响，探讨其改善 MIRI 后心室重构的潜在机制。
40 只雄性 SD 大鼠随机分为 4 组：假手术组、模型组、电针组和药物（沙库巴曲缬沙坦，LCZ696）组，每组 10 只。通过结扎左前降支和再灌注建立 MIRI 模型。电针（2 Hz/100 Hz，2 mA）应用于双侧“内关”（PC6）20 min，隔天一次，共 21 d。药物组大鼠给予 LCZ696（60 mg·kg·d）灌胃。干预后，采用超声心动图检测左心室射血分数（EF）和短轴缩短率（FS），酶联免疫吸附法检测血清肿瘤坏死因子-α（TNF-α）、血管细胞黏附分子-1（VCAM-1）和细胞间黏附分子-1（ICAM-1）含量。HE 染色观察心肌组织病理变化。Masson 染色评估心肌胶原沉积和心肌纤维化程度。采用实时定量 PCR 检测心肌组织中胶原Ⅰ和Ⅲ和结缔组织生长因子（CTGF）的 mRNA 表达水平，Western blot 检测 IL-1β和 IL-18 的表达水平。
与假手术组相比，模型组左心室 EF 和 FS 水平明显降低（<0.001），血清 TNF-α、VCAM-1 和 ICAM-1 含量、心肌纤维化面积比例、心肌胶原Ⅰ、胶原Ⅲ和 CTGF mRNA 表达水平以及 IL-1β和 IL-18 表达水平均明显升高（<0.001，<0.000 1，<0.05，<0.01）。与模型组相比，药物组和电针组 EF 和 FS 水平明显升高（<0.01），血清 TNF-α、VCAM-1 和 ICAM-1 含量、心肌纤维化面积比例、心肌胶原Ⅰ、胶原Ⅲ和 CTGF mRNA 表达水平以及 IL-1β和 IL-18 表达水平均明显下调（<0.001，<0.01，<0.05）。电针组和药物组上述各项指标比较差异均无统计学意义。
电针可改善 MIRI 大鼠左心室纤维化和功能，延缓或逆转心室重构，这可能与其下调心肌炎症反应和心肌胶原Ⅰ、胶原Ⅲ和 CTGF mRNA 表达水平有关。
