Department of Psychology and Sociology of Health and Public Health, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland.
Department of Clinical Laboratory Diagnostics, Medical University of Bialystok, Bialystok, Poland.
Front Immunol. 2024 Jan 4;14:1320362. doi: 10.3389/fimmu.2023.1320362. eCollection 2023.
The aim of the present study was to assess differences in the serum levels of chemokines and growth factors (GFs) between COVID-19 patients and healthy controls. The diagnostic utility of the analyzed proteins for monitoring the severity of the SARS-CoV- 2 infection based on the patients' MEWS scores was also assessed.
The serum levels of chemokines and growth factors were analyzed in hospitalized COVID-19 patients (50 women, 50 men) with the use of the Bio-Plex Pro™ Human Cytokine Screening Panel (Biorad) and the Bio-Plex Multiplex system.
The study demonstrated that serum levels of MIP-1α, RANTES, Eotaxin, CTACK, GRO-α, IP-10, MIG, basic-FGF, HGF, SCGF-β, G-CSF, M-CSF, SCF, MIF, LIF, and TRAIL were significant higher in COVID-19 patients than in the control group. The concentrations of CTACK, GRO-α, IP-10, MIG, basic-FGF, HGF, PDGF- BB, GM-CSF, SCF, LIF, and TRAIL were higher in asymptomatic/mildly symptomatic COVID-19 patients (stage 1) and COVID-19 patients with pneumonia without respiratory failure (stage 2). The receiver operating characteristic (ROC) analysis revealed that IP-10, MIF, MIG, and basic-FGF differentiated patients with COVID-19 from healthy controls with the highest sensitivity and specificity, whereas GM-CSF, basic-FGF, and MIG differentiated asymptomatic/mildly symptomatic COVID-19 patients (stage 1) from COVID-19 patients with pneumonia without respiratory failure (stage 2) with the highest sensitivity and specificity.
MIG, basic-FGF, and GM-CSF can be useful biomarkers for monitoring disease severity in patients with COVID-19.
本研究旨在评估 COVID-19 患者与健康对照者之间趋化因子和生长因子（GFs）血清水平的差异。还评估了基于患者 MEWS 评分分析的蛋白质对监测 SARS-CoV-2 感染严重程度的诊断效用。
使用 Bio-Plex Pro™ 人类细胞因子筛选试剂盒（Biorad）和 Bio-Plex 多重系统，分析了住院 COVID-19 患者（50 名女性，50 名男性）的趋化因子和生长因子的血清水平。
研究表明，与对照组相比，COVID-19 患者血清中 MIP-1α、RANTES、Eotaxin、CTACK、GRO-α、IP-10、MIG、basic-FGF、HGF、SCGF-β、G-CSF、M-CSF、SCF、MIF、LIF 和 TRAIL 的水平显著升高。无症状/轻度症状 COVID-19 患者（第 1 期）和无呼吸衰竭肺炎 COVID-19 患者（第 2 期）血清中 CTACK、GRO-α、IP-10、MIG、basic-FGF、HGF、PDGF- BB、GM-CSF、SCF、LIF 和 TRAIL 浓度较高。受试者工作特征（ROC）分析表明，IP-10、MIF、MIG 和 basic-FGF 对 COVID-19 患者与健康对照者的区分具有最高的敏感性和特异性，而 GM-CSF、basic-FGF 和 MIG 对无症状/轻度症状 COVID-19 患者（第 1 期）与无呼吸衰竭肺炎 COVID-19 患者（第 2 期）的区分具有最高的敏感性和特异性。
MIG、basic-FGF 和 GM-CSF 可作为监测 COVID-19 患者疾病严重程度的有用生物标志物。
