探索肠道微生物群与斑秃之间的联系：两样本孟德尔随机分析。

Exploring the link between gut microbiota and alopecia areata: a two-sample Mendelian randomization analysis.

机构信息

School of Medicine, Zhejiang University, Hangzhou, People's Republic of China.

Department of Dermatology, Hangzhou Third People's Hospital, Affiliated Hangzhou Dermatology Hospital, Zhejiang University School of Medicine, Hangzhou Third Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou, People's Republic of China.

出版信息

Int J Dermatol. 2024 May;63(5):597-603. doi: 10.1111/ijd.17032. Epub 2024 Jan 19.

DOI:10.1111/ijd.17032

PMID:38240406

Abstract

BACKGROUND

While observational studies have suggested a link between gut microbiota diversity and alopecia areata (AA), the causal relationship remains unclear.

METHODS

We leveraged data from the MiBioGen and FinnGen consortiums' Genome-wide association studies (GWAS) encompassing gut microbiota (n = 13,266) and AA (n = 211,428) datasets. A comprehensive Mendelian randomization (MR) and reverse MR approach were employed, utilizing five statistical methods to evaluate causality. Sensitivity analyses were also conducted to corroborate the MR results.

RESULTS

Inverse variance weighted (IVW) analysis indicated a protective effect against AA from Butyricimonas (OR = 0.37, 95% CI: 0.18-0.77, P = 0.01), Enterorhabdus (OR = 0.40, 95% CI: 0.16-0.95, P = 0.04), Eubacterium (xylanophilum group) (OR = 0.36, 95% CI: 0.15-0.84, P = 0.02), and Phascolarctobacterium (OR = 0.37, 95% CI: 0.15-0.91, P = 0.03), while Ruminococcaceae UCG003 posed as a risk factor (OR = 2.79, 95% CI: 1.27-6.14, P = 0.01). Reverse MR showed no significant causal link between AA and gut microbiota, with no significant heterogeneity or horizontal pleiotropy.

CONCLUSIONS

Our analysis suggests probable causality between certain gut microbiota and AA, shedding light on its pathogenesis and potential intervention strategies.

摘要

背景

虽然观察性研究表明肠道微生物多样性与斑秃（AA）之间存在关联，但因果关系尚不清楚。

方法

我们利用 MiBioGen 和 FinnGen 联盟的全基因组关联研究（GWAS）的数据，其中包括肠道微生物（n=13266）和 AA（n=211428）数据集。采用综合孟德尔随机化（MR）和反向 MR 方法，利用五种统计方法评估因果关系。还进行了敏感性分析以验证 MR 结果。

结果

反向方差加权（IVW）分析表明，丁酸单胞菌（OR=0.37，95%CI：0.18-0.77，P=0.01）、肠杆菌（OR=0.40，95%CI：0.16-0.95，P=0.04）、真杆菌（木聚糖分解菌组）（OR=0.36，95%CI：0.15-0.84，P=0.02）和 Phascolarctobacterium（OR=0.37，95%CI：0.15-0.91，P=0.03）对 AA 有保护作用，而 Ruminococcaceae UCG003 则是一个风险因素（OR=2.79，95%CI：1.27-6.14，P=0.01）。反向 MR 显示 AA 与肠道微生物之间没有显著的因果关系，没有显著的异质性或水平多效性。