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用于艰难梭菌分型的单克隆抗体集合？

A monoclonal antibody collection for C. difficile typing ?

作者信息

Hunault Lise, England Patrick, Barbut Frédéric, Iannascoli Bruno, Godon Ophélie, Déjardin François, Thomas Christophe, Dupuy Bruno, Guo Chunguang, Macdonald Lynn, Gorochov Guy, Sterlin Delphine, Bruhns Pierre

机构信息

Antibodies in Therapy and Pathology, Institut Pasteur, Université Paris Cité, INSERM UMR1222, 75015, Paris, France.

Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Sorbonne Université, INSERM, CNRS, 75013, Paris, France.

出版信息

Gut Pathog. 2024 Jan 19;16(1):4. doi: 10.1186/s13099-023-00592-7.

DOI:10.1186/s13099-023-00592-7
PMID:38243246
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10797914/
Abstract

Clostridioides difficile is the leading cause of antibiotic-associated diarrhea and pseudomembranous colitis in adults. Various C. difficile strains circulate currently, associated with different outcomes and antibiotic resistance profiles. However, most studies still focus on the reference strain 630 that does not circulate anymore, partly due to the lack of immunological tools to study current clinically important C. difficile PCR ribotypes. The goal of this study was to generate monoclonal antibodies recognizing various epidemic ribotypes of C. difficile. To do so, we immunized mice expressing human variable antibody genes with the Low Molecular Weight (LMW) subunit of the surface layer protein SlpA from various C. difficile strains. Monoclonal antibodies purified from hybridomas bound LMW with high-affinity and whole bacteria from current C. difficile ribotypes with different cross-specificities. This first collection of anti-C. difficile mAbs represent valuable tools for basic and clinical research.

摘要

艰难梭菌是成人抗生素相关性腹泻和伪膜性结肠炎的主要病因。目前有多种艰难梭菌菌株在传播，与不同的结果和抗生素耐药谱相关。然而，大多数研究仍集中在已不再传播的参考菌株630上，部分原因是缺乏用于研究当前临床上重要的艰难梭菌PCR核糖体分型的免疫学工具。本研究的目的是生成识别多种艰难梭菌流行核糖体分型的单克隆抗体。为此，我们用来自多种艰难梭菌菌株的表层蛋白SlpA的低分子量（LMW）亚基免疫表达人可变抗体基因的小鼠。从杂交瘤中纯化的单克隆抗体以高亲和力结合LMW，并与来自当前具有不同交叉特异性的艰难梭菌核糖体分型的全菌结合。这第一批抗艰难梭菌单克隆抗体是基础研究和临床研究的宝贵工具。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be12/10797914/aaa32e80418f/13099_2023_592_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be12/10797914/8c2d8e7db907/13099_2023_592_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be12/10797914/a54f59988e05/13099_2023_592_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be12/10797914/3bce0d2f406d/13099_2023_592_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be12/10797914/aaa32e80418f/13099_2023_592_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be12/10797914/8c2d8e7db907/13099_2023_592_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be12/10797914/a54f59988e05/13099_2023_592_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be12/10797914/3bce0d2f406d/13099_2023_592_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be12/10797914/aaa32e80418f/13099_2023_592_Fig4_HTML.jpg

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本文引用的文献

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Genomic epidemiology and transmission dynamics of recurrent Clostridioides difficile infection in Western Australia.澳大利亚西部复发性艰难梭菌感染的基因组流行病学和传播动态。
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Structure and assembly of the S-layer in C. difficile.艰难梭菌中 S 层的结构与组装。
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Practical observations on the use of fluorescent reporter systems in Clostridioides difficile.荧光报告系统在艰难梭菌中的应用的实际观察。
Antonie Van Leeuwenhoek. 2022 Feb;115(2):297-323. doi: 10.1007/s10482-021-01691-8. Epub 2022 Jan 18.
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The anti-IgE mAb omalizumab induces adverse reactions by engaging Fcγ receptors.抗 IgE 单克隆抗体奥马珠单抗通过结合 Fcγ 受体诱导不良反应。
J Clin Invest. 2020 Mar 2;130(3):1330-1335. doi: 10.1172/JCI129697.
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Two-year analysis of Clostridium difficile ribotypes associated with increased severity.与严重程度增加相关的艰难梭菌核糖体分型的两年分析。
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