Nyberg G, Mårtensson E
Ther Drug Monit. 1986;8(4):478-82. doi: 10.1097/00007691-198612000-00019.
The effects were tested of eight common types of blood collection tubes and two types of "plasma separators" on the stability of the tricyclic antidepressants amitriptyline, imipramine, clomipramine, and their monodemethylated metabolites in venous blood samples. Although EDTA-containing Venoject lavender and Vacutainer lavender tubes seemed to give the most stable plasma samples, and Venoject red the most stable serum samples, the differences were too small to have practical consequences. Vacutainer royal blue collection tubes gave significant losses of greater than 20% of some of the substances. The tubes with serum separator gel or filter proved unsuitable, since they were responsible for losses of greater than 40%. The losses were not caused by redistribution between blood cells and plasma but occurred mainly as a result of contact between the contents and the caps of the tubes. Experiments with freezing, thawing, and storage of samples showed that freshly sampled blood could be stored at room temperature for 24 h in Venoject green tubes without significant losses. Serum samples could be stored at refrigerator temperature for 4 weeks without important losses. Freezing, thawing, and storage at -20 degrees C did not influence the serum or plasma concentrations.
测试了八种常见类型的采血管和两种类型的“血浆分离器”对静脉血样本中三环类抗抑郁药阿米替林、丙咪嗪、氯米帕明及其单去甲基代谢物稳定性的影响。尽管含乙二胺四乙酸（EDTA）的威高洁淡紫色管和贝克曼库尔特淡紫色管似乎能提供最稳定的血浆样本，而威高洁红色管能提供最稳定的血清样本，但差异过小，不具有实际影响。贝克曼库尔特宝蓝色采血管导致某些物质损失超过20%，损失显著。含血清分离胶或过滤器的采血管被证明不合适，因为它们导致的损失超过40%。这些损失并非由血细胞与血浆之间的重新分布引起，而是主要由于管内物质与管帽接触所致。样本的冷冻、解冻和储存实验表明，新鲜采集的血液在威高洁绿色管中于室温下保存24小时，不会有显著损失。血清样本在冰箱温度下保存4周，也不会有重大损失。在-20℃下冷冻、解冻和储存不会影响血清或血浆浓度。
