辐照中性粒细胞中氧化产物的形成。流式细胞术分析。

Oxidative product formation in irradiated neutrophils. A flow cytometric analysis.

作者信息

Wolber R A, Duque R E, Robinson J P, Oberman H A

出版信息

Transfusion. 1987 Mar-Apr;27(2):167-70. doi: 10.1046/j.1537-2995.1987.27287150192.x.

DOI:10.1046/j.1537-2995.1987.27287150192.x

Abstract

The effect of irradiation on neutrophil oxidative function was evaluated using a flow cytometric assay of intracellular hydrogen peroxide (H2O2) production. This assay quantitates the H2O2-dependent conversion of the nonfluorescent compound, 2'-7'-dichlorofluorescein (DCFH), into fluorescent 2'-7'-dichlorofluorescein (DCF) on a single-cell basis. Intracellular H2O2 production in response to stimulation with phorbol myristate acetate was not affected by neutrophil irradiation at doses up to 2500 rad. In addition, irradiation of intracellular DCFH and aqueous 2'-7'-dichlorofluorescein diacetate (DCFH-DA) resulted in DCF production, which suggested that oxidative molecules produced by aqueous radiolysis were detected by this assay. This study indicates that radiation doses of 1500 to 2500 rad, which are sufficient to prevent induction of graft-versus-host disease by transfused blood components, are not deleterious to neutrophil oxidative metabolism.

摘要

使用细胞内过氧化氢（H2O2）产生的流式细胞术测定法评估辐射对中性粒细胞氧化功能的影响。该测定法在单细胞基础上定量非荧光化合物2'-7'-二氯荧光素（DCFH）向荧光性2'-7'-二氯荧光素（DCF）的H2O2依赖性转化。用佛波酯肉豆蔻酸酯乙酸盐刺激后，高达2500拉德剂量的中性粒细胞辐射对细胞内H2O2产生没有影响。此外，细胞内DCFH和水性2'-7'-二氯荧光素二乙酸酯（DCFH-DA）的辐射导致DCF产生，这表明该测定法检测到了水性辐射分解产生的氧化分子。本研究表明，1500至2500拉德的辐射剂量足以防止输血成分诱发移植物抗宿主病，对中性粒细胞氧化代谢无害。