IFNφ1 和 IFNφ4 在斑马鱼中表现出不同的抗病毒活性。
Distinct antiviral activities of IFNφ1 and IFNφ4 in zebrafish.
机构信息
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266200, China.
出版信息
Fish Shellfish Immunol. 2024 Mar;146:109396. doi: 10.1016/j.fsi.2024.109396. Epub 2024 Jan 18.
Interferons (IFNs) are a group of secreted cytokines that play a crucial role in antiviral immunity. Type I IFNs display functional disparities. In teleosts, type I IFNs are categorized into two subgroups containing one or two pairs of disulfide bonds. However, their functional differences have not been fully elucidated. In this study, we comparatively characterized the antiviral activities of zebrafish IFNφ1 and IFNφ4 belonging to the group I type I IFNs. It was found that ifnφ1 and ifnφ4 were differentially modulated during viral infection. Although both IFNφ1 and IFNφ4 activated JAK-STAT signaling pathway via CRFB1/CRFB5 receptor complex, IFNφ4 was less potent in inducing phosphorylation of STAT1a, STAT1b and STAT2 and the expression of antiviral genes than IFNφ1, thereby conferring weaker antiviral resistance of target cells. Taken together, our results provide insights into the functional divergence of type I IFNs in lower vertebrates.
干扰素（IFNs）是一组分泌细胞因子，在抗病毒免疫中发挥着关键作用。I 型 IFNs 具有不同的功能。在硬骨鱼中，I 型 IFNs 分为两个亚组，包含一对或两对二硫键。然而，它们的功能差异尚未完全阐明。在这项研究中，我们比较了属于 I 型 IFN 的斑马鱼 IFNφ1 和 IFNφ4 的抗病毒活性。结果发现，ifnφ1 和 ifnφ4 在病毒感染过程中被差异调控。尽管 IFNφ1 和 IFNφ4 通过 CRFB1/CRFB5 受体复合物激活 JAK-STAT 信号通路，但 IFNφ4 在诱导 STAT1a、STAT1b 和 STAT2 的磷酸化以及抗病毒基因的表达方面比 IFNφ1 弱，从而赋予靶细胞较弱的抗病毒抗性。总之，我们的结果为研究低等脊椎动物中 I 型 IFNs 的功能分化提供了新的思路。
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