Life Science Institute, Jinzhou Medical University, Jinzhou, China; Department of Clinical Laboratory, Lequn Branch, The First Hospital of Jilin University, Changchun, Jilin, China.
Life Science Institute, Jinzhou Medical University, Jinzhou, China.
Int Immunopharmacol. 2024 Feb 15;128:111563. doi: 10.1016/j.intimp.2024.111563. Epub 2024 Jan 20.
IL-10 is a pleiotropic cytokine that plays a significant role in antiviral and antitumor immunity. Potent CD8 T cells express IL-10 after stimulation by strong TCR signaling, which promotes the killing effect of CD8 T cells. However, the regulation of IL-10 expression in CD8 T cells and its signaling pathway to enhance CD8 T cell function are largely unknown. In this study, we investigated the JAK-STAT signaling molecules that regulate IL-10 expression in CD8 T cells and the JAK-STAT signaling pathway that IL-10 enhances the function of CD8 T cells through its receptor, using small molecule inhibitors and CRISPR-Cas9 gene editing. Our findings provide new insights and a theoretical basis for the immunotherapy of tumors.
IL-10 是一种多功能细胞因子，在抗病毒和抗肿瘤免疫中发挥重要作用。在强烈的 TCR 信号刺激下，有效的 CD8 T 细胞表达 IL-10，这促进了 CD8 T 细胞的杀伤作用。然而，CD8 T 细胞中 IL-10 的表达调控及其信号通路增强 CD8 T 细胞功能在很大程度上仍是未知的。在这项研究中，我们使用小分子抑制剂和 CRISPR-Cas9 基因编辑技术，研究了调节 CD8 T 细胞中 IL-10 表达的 JAK-STAT 信号分子以及 IL-10 通过其受体增强 CD8 T 细胞功能的 JAK-STAT 信号通路。我们的研究结果为肿瘤的免疫治疗提供了新的见解和理论基础。
