Li Siqi, Liu Tianyang, Liu Cheng, Sun Donglei, Yan Qin, Gao Dengzhou, Zhang Zongxiao
Department of Military Oceanography and Hydrography and Cartography, Dalian Naval Academy, Dalian, China.
Shandong Key Laboratory of Eco-Environmental Science for the Yellow River Delta, Binzhou University, Binzhou, Shandong, China.
Front Microbiol. 2024 Jan 5;14:1341564. doi: 10.3389/fmicb.2023.1341564. eCollection 2023.
Here we investigated the potential impacts of soil inorganic nitrogen (SIN) content on the phylogenetic characteristics and ecological functions of soil bacterial communities in estuarine intertidal zones in China, aiming to comprehend the response mechanism of soil microorganisms to variations in SIN content within estuarine wetlands. Our results show that SIN in estuarine areas has a significant spatiotemporal variation on spatial and seasonal scales, in this study and is significantly associated with the phylogenetic diversity and phylogenetic turnover of soil bacterial communities. In addition, the results of the metagenomic analysis showed that the relative abundance of nitrogen-cycling functional genes in bacterial communities did not differ significantly in sampling sites and seasons, and weakly correlated with SIN content. Further, the results based on structural equation modeling (SEM) analysis showed that SIN directly and significantly regulated the phylogenetic characteristics of bacterial communities, thereby indirectly affecting the potential of bacterial nitrogen metabolism. This study emphasizes the key influence of SIN variations on the phylogenetic dissimilarity in soil bacterial communities. Moreover, although there was a weak direct relationship between the functional characteristics of the bacterial nitrogen metabolism and SIN content, the spatiotemporal variation of bacterial nitrogen metabolic potential may be indirectly regulated by SIN content by influencing the phylogenetic diversity in bacterial communities. Our study unravels the pivotal mechanisms through which SIN content influences bacterial communities, thereby offering novel insights into the microbial intricacies governing nitrogen metabolism within estuaries.
在此，我们研究了土壤无机氮（SIN）含量对中国河口潮间带土壤细菌群落系统发育特征和生态功能的潜在影响，旨在了解河口湿地土壤微生物对SIN含量变化的响应机制。我们的研究结果表明，河口地区的SIN在空间和季节尺度上具有显著的时空变化，并且在本研究中与土壤细菌群落的系统发育多样性和系统发育周转率显著相关。此外，宏基因组分析结果表明，细菌群落中氮循环功能基因的相对丰度在采样地点和季节上没有显著差异，并且与SIN含量弱相关。进一步地，基于结构方程模型（SEM）分析的结果表明，SIN直接且显著地调节了细菌群落的系统发育特征，从而间接影响了细菌氮代谢的潜力。本研究强调了SIN变化对土壤细菌群落系统发育差异的关键影响。此外，尽管细菌氮代谢的功能特征与SIN含量之间存在较弱的直接关系，但细菌氮代谢潜力的时空变化可能通过影响细菌群落的系统发育多样性而受到SIN含量的间接调节。我们的研究揭示了SIN含量影响细菌群落的关键机制，从而为河口内控制氮代谢的微生物复杂性提供了新的见解。
