Department of Life Science and Applied Chemistry, Graduate School of Science, Nagoya Institute of Technology, Gokiso-cho, Showa-ku, Nagoya 466-8555, Japan.
Department of Applied Chemistry, Aichi Institute of Technology, 1247 Yachigusa, Yakusa-cho, Toyota 470-0392, Japan.
Langmuir. 2024 Feb 6;40(5):2632-2645. doi: 10.1021/acs.langmuir.3c03084. Epub 2024 Jan 22.
Four Fe complexes of typical artificial siderophore ligands containing catecholate and/or hydroxamate groups of tricatecholate, biscatecholate-monohydroxamate, monocatecholate-bishydroxamate, and trihydroxamate type artificial siderophores (K[], K[], K[], and []) were modified on Au substrate surfaces. Their abilities to adsorb microorganisms were investigated using scanning electron microscopy, quartz crystal microbalance, and AC impedance methods. The artificial siderophore-iron complexes modified on Au substrates (/Au, /Au, /Au, and /Au) showed the selective immobilization behavior for various microorganisms, depending on the structural features of the artificial siderophores (the number of catecholate and hydroxamate arms). Their specificities corresponded well with the structural characteristics of natural siderophores released by microorganisms and used for Fe ion uptake. These findings suggest that they were generated via specific interactions between the artificial siderophore-Fe complexes and the receptors on microorganism surfaces. Our observations revealed that the /Au systems may be potentially used as effective microbe-capturing probes that can enable rapid and simple detection and identification of various microorganisms.
四种含有邻苯二酚和/或羟肟酸基团的典型人工铁载体配体的 Fe 配合物（三邻苯二酚、双邻苯二酚-单羟肟酸、单邻苯二酚-双羟肟酸和三羟肟酸型人工铁载体）（K[]、K[]、K[]和 []) 被修饰在 Au 基底表面上。使用扫描电子显微镜、石英晶体微天平（QCM）和交流阻抗法研究了它们吸附微生物的能力。在 Au 基底上修饰的人工铁载体-铁配合物（/Au、/Au、/Au 和 /Au）表现出对各种微生物的选择性固定化行为，这取决于人工铁载体的结构特征（邻苯二酚和羟肟酸臂的数量）。它们的特异性与微生物释放的天然铁载体的结构特征很好地对应，这些天然铁载体用于 Fe 离子的摄取。这些发现表明，它们是通过人工铁载体-Fe 配合物与微生物表面受体之间的特异性相互作用产生的。我们的观察结果表明，/Au 系统可能潜在地用作有效的微生物捕获探针，能够快速、简单地检测和识别各种微生物。
