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优化稻壳转化为增值产品的生物转化过程:D-塔格糖、生物乙醇和乳酸。

Optimizing bioconversion processes of rice husk into value-added products: D-psicose, bioethanol, and lactic acid.

机构信息

Bio-energy Research Center, Chonnam National University, Gwangju 61186, Republic of Korea.

School of Biotechology, Tan Tao University, Long An 82000, Vietnam.

出版信息

Bioresour Technol. 2024 Mar;395:130363. doi: 10.1016/j.biortech.2024.130363. Epub 2024 Jan 20.

DOI:10.1016/j.biortech.2024.130363
PMID:38253244
Abstract

Rice husk, rich carbon content, is an agricultural waste produced globally at an amount of 120 million tons annually, and it has high potential as a biorefinery feedstock. Herein, we investigated the feasibility of producing various products as D-psicose, bioethanol and lactic acid from rice husk (RH) through a biorefinery process. Alkali-hydrogen peroxide-acetic acid pretreatment of RH effectively removed lignin and silica, resulting in enzymatic hydrolysis yield of approximately 86.3% under optimal hydrolysis conditions. By using xylose isomerase as well as D-psicose-3-epimerase with borate, glucose present in the RH hydrolysate was converted into D-psicose with a 40.6% conversion yield in the presence of borate. Furthermore, bioethanol (85.4%) and lactic acid (92.5%) were successfully produced from the RH hydrolysate. This study confirmed the high potential of RH as a biorefinery feedstock, and it is expected that various platform chemicals and value-added products can be produced using RH.

摘要

稻壳富含碳元素,是全球每年产生的 1.2 亿吨农业废弃物,具有很高的作为生物炼制原料的潜力。在此,我们通过生物炼制工艺研究了从稻壳(RH)生产各种产品(如 D-阿洛酮糖、生物乙醇和乳酸)的可行性。RH 的碱-过氧化氢-乙酸预处理有效地去除了木质素和二氧化硅,在最佳水解条件下,酶水解产率约为 86.3%。通过使用木糖异构酶和硼酸存在下的 D-阿洛酮糖-3-差向异构酶,RH 水解液中的葡萄糖在硼酸存在下转化为 D-阿洛酮糖,转化率为 40.6%。此外,还可以从 RH 水解液中成功生产出 85.4%的生物乙醇和 92.5%的乳酸。本研究证实了 RH 作为生物炼制原料的巨大潜力,预计可以使用 RH 生产各种平台化学品和增值产品。

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