Xie Yanjun, Huang Yi, Stevenson Hugo C S, Yin Li, Zhang Kaijie, Islam Zain Husain, Marcum William Aaron, Johnston Campbell, Hoyt Nicholas, Kent Eric William, Wang Bowen, Hossack John A
Department of Biomedical Engineering, University of Virginia, Charlottesville, VA 22908, USA.
Department of Surgery, School of Medicine, University of Virginia, Charlottesville, VA 22908, USA.
Biomedicines. 2024 Jan 16;12(1):0. doi: 10.3390/biomedicines12010200.
Deep vein thrombosis (DVT) is a life-threatening condition that can lead to its sequelae pulmonary embolism (PE) or post-thrombotic syndrome (PTS). Murine models of DVT are frequently used in early-stage disease research and to assess potential therapies. This creates the need for the reliable and easy quantification of blood clots. In this paper, we present a novel high-frequency 3D ultrasound approach for the quantitative evaluation of the volume of DVT in an in vitro model and an in vivo murine model. The proposed method involves the use of a high-resolution ultrasound acquisition system and semiautomatic segmentation of the clot. The measured 3D volume of blood clots was validated to be correlated with in vitro blood clot weights with an R2 of 0.89. Additionally, the method was confirmed with an R2 of 0.91 in the in vivo mouse model with a cylindrical volume from macroscopic measurement. We anticipate that the proposed method will be useful in pharmacological or therapeutic studies in murine models of DVT.
深静脉血栓形成（DVT）是一种危及生命的疾病，可导致其后遗症肺栓塞（PE）或血栓后综合征（PTS）。DVT的小鼠模型常用于早期疾病研究和评估潜在疗法。这就需要对血凝块进行可靠且简便的定量分析。在本文中，我们提出了一种新颖的高频三维超声方法，用于在体外模型和体内小鼠模型中对DVT体积进行定量评估。所提出的方法涉及使用高分辨率超声采集系统和血凝块的半自动分割。测得的血凝块三维体积经验证与体外血凝块重量相关，R2为0.89。此外，该方法在体内小鼠模型中通过宏观测量的圆柱体积得到了验证，R2为0.91。我们预计所提出的方法将在DVT小鼠模型的药理学或治疗研究中发挥作用。
