Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou 510642, China.
Int J Mol Sci. 2024 Jan 17;25(2):1142. doi: 10.3390/ijms25021142.
Real-time quantitative polymerase chain reaction (qRT-PCR) has been widely used in gene expression analyses due to its advantages of sensitivity, accuracy and high throughput. The stability of internal reference genes has progressively emerged as a major factor affecting the precision of qRT-PCR results. However, the stability of the expression of the reference genes needs to be determined further in different cells or organs, physiological and experimental conditions. Methods for evaluating these candidate internal reference genes have also evolved from simple single software evaluation to more reliable and accurate internal reference gene evaluation by combining different software tools in a comprehensive analysis. This study intends to provide a definitive reference for upcoming research that will be conducted on fruit trees. The primary focus of this review is to summarize the research progress in recent years regarding the selection and stability analysis of candidate reference genes for different fruit trees.
实时定量聚合酶链反应（qRT-PCR）由于其灵敏度、准确性和高通量的优势，已广泛应用于基因表达分析。内参基因的稳定性逐渐成为影响 qRT-PCR 结果准确性的主要因素。然而，在不同的细胞或器官、生理和实验条件下，参考基因的表达稳定性需要进一步确定。评估这些候选内参基因的方法也从简单的单一软件评估演变为通过综合分析结合不同的软件工具进行更可靠和准确的内参基因评估。本研究旨在为即将进行的果树研究提供明确的参考。本综述的主要重点是总结近年来不同果树候选内参基因的选择和稳定性分析方面的研究进展。
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