In-ear acquisition of physiological signals, such as electromyography (EMG), electrooculography (EOG), electroencephalography (EEG), and electrocardiography (ECG), is a promising approach to mobile health (mHealth) due to its non-invasive and user-friendly nature. By providing a convenient and comfortable means of physiological signal monitoring, in-ear signal acquisition could potentially increase patient compliance and engagement with mHealth applications. The development of reliable and comfortable soft dry in-ear electrode systems could, therefore, have significant implications for both mHealth and human-machine interface (HMI) applications. This research evaluates the quality of the ECG signal obtained with soft dry electrodes inserted in the ear canal. An earplug with six soft dry electrodes distributed around its perimeter was designed for this study, allowing for the analysis of the signal coming from each electrode independently with respect to a common reference placed at different positions on the body of the participants. An analysis of the signals in comparison with a reference signal measured on the upper right chest (RA) and lower left chest (LL) was performed. The results show three typical behaviors for the in-ear electrodes. Some electrodes have a high correlation with the reference signal directly after inserting the earplug, other electrodes need a settling time of typically 1-3 min, and finally, others never have a high correlation. The SoftPulse electrodes used in this research have been proven to be perfectly capable of measuring physiological signals, paving the way for their use in mHealth or HMI applications. The use of multiple electrodes distributed in the ear canal has the advantage of allowing a more reliable acquisition by intelligently selecting the signal acquisition locations or allowing a better spatial resolution for certain applications by processing these signals independently.