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探索在多微生物生物膜形成过程中与/之间相互作用的复杂性。 （你提供的原文中“and /”表述不完整，可能影响准确理解，以上是按现有内容翻译。）

Exploring the Complexity of the Interaction between and / in the Formation of Polymicrobial Biofilms.

作者信息

Belizario Jenyffie A, Bila Níura M, Vaso Carolina O, Costa-Orlandi Caroline B, Mendonça Matheus B, Fusco-Almeida Ana M, Pires Regina H, Mendes-Giannini Maria José S

机构信息

Department of Clinical Analysis, School of Pharmaceutical Sciences, São Paulo State University (U.N.E.S.P.), São Paulo 14800-903, Brazil.

Department of Para-Clinic, School of Veterinary, Eduardo Mondlane University (UEM), Maputo 257, Mozambique.

出版信息

Microorganisms. 2024 Jan 18;12(1):191. doi: 10.3390/microorganisms12010191.

DOI:10.3390/microorganisms12010191
PMID:38258017
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10820507/
Abstract

Dermatophytes associated with bacteria can lead to severe, difficult-to-treat infections and contribute to chronic infections. , , and can form biofilms influenced by nutrient availability. This study investigated biofilm formation by these species by utilizing diverse culture media and different time points. These biofilms were studied through scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), biomass, metabolic activity, and colony-forming units (CFUs). The results revealed that mixed biofilms exhibited high biomass and metabolic activity when cultivated in the brain heart infusion (BHI) medium. Both bacterial species formed mature biofilms with within 72 h, irrespective of media. The timing of bacterial inoculation was pivotal in influencing biomass and metabolic activity. 's development within mixed biofilms depended on bacterial addition timing, while pre-adhesion influenced fungal growth. Bacterial communities prevailed initially, while fungi dominated later in the mixed biofilms. CLSM revealed 363 μm thick biofilms with septate, well-developed hyphae; (177 μm) and (178 μm) biofilms showed primarily cocci. Mixed biofilms matched 's thickness when associated with (369 μm), with few hyphae initially. Understanding and Staphylococcal interactions in biofilms advances antimicrobial resistance and disease progression knowledge.

摘要

与细菌相关的皮肤癣菌可导致严重的、难以治疗的感染，并促成慢性感染。 、 和 可形成受营养物质可用性影响的生物膜。本研究通过使用不同的培养基和不同的时间点来研究这些菌种的生物膜形成情况。通过扫描电子显微镜（SEM）、共聚焦激光扫描显微镜（CLSM）、生物量、代谢活性和菌落形成单位（CFU）对这些生物膜进行了研究。结果显示，当在脑心浸液（BHI）培养基中培养时，混合生物膜表现出高生物量和代谢活性。两种细菌菌种在72小时内均形成了成熟的生物膜，与培养基无关。细菌接种的时间对于影响生物量和代谢活性至关重要。 在混合生物膜中的发育取决于细菌添加时间，而预粘附影响真菌生长。在混合生物膜中，细菌群落最初占主导，而真菌后来占主导。CLSM显示 生物膜厚度为363μm，有分隔的、发育良好的菌丝； （177μm）和 （178μm）生物膜主要显示球菌。当与 相关时，混合生物膜的厚度与 匹配（369μm），最初几乎没有菌丝。了解生物膜中 和葡萄球菌的相互作用有助于增进对抗菌素耐药性和疾病进展的认识。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/ccc42ba9ad77/microorganisms-12-00191-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/a57e6301f01e/microorganisms-12-00191-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/605953fd73f9/microorganisms-12-00191-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/ba4ee4cdcf43/microorganisms-12-00191-g003.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/70d915cde441/microorganisms-12-00191-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/65306ea95a04/microorganisms-12-00191-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/8f15f6e71bf6/microorganisms-12-00191-g007a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/241fa7e68d61/microorganisms-12-00191-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/2d2594347f7e/microorganisms-12-00191-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/ce7a34ad8546/microorganisms-12-00191-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/396da8056b17/microorganisms-12-00191-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/2f7b5f2bee0a/microorganisms-12-00191-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/ccc42ba9ad77/microorganisms-12-00191-g013.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/a57e6301f01e/microorganisms-12-00191-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/605953fd73f9/microorganisms-12-00191-g002a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/ba4ee4cdcf43/microorganisms-12-00191-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/d75f4f27523d/microorganisms-12-00191-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/70d915cde441/microorganisms-12-00191-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/65306ea95a04/microorganisms-12-00191-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/8f15f6e71bf6/microorganisms-12-00191-g007a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/241fa7e68d61/microorganisms-12-00191-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/2d2594347f7e/microorganisms-12-00191-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/ce7a34ad8546/microorganisms-12-00191-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/396da8056b17/microorganisms-12-00191-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/2f7b5f2bee0a/microorganisms-12-00191-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bed4/10820507/ccc42ba9ad77/microorganisms-12-00191-g013.jpg

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