Stein T P, Hoyt R W, Settle R G, O'Toole M, Hiller W D
Am J Clin Nutr. 1987 Mar;45(3):534-9. doi: 10.1093/ajcn/45.3.534.
Energy expenditure of four subjects was measured by the doubly-labelled-water (2H2 18O) method to determine if energy expenditure could be determined over short periods. Three subjects were studied while they performed 8 h of heavy exercise in a laboratory environment. Urine and blood samples were taken before and after exercise. Estimated energy expended during 8 h of high-intensity exercise (55% VO2 max) for three subjects was 757 +/- 118 kcal/h by the doubly-labelled-water method using urine and a two-point calculation, which compared favorably with 735 +/- 82 kcal/h obtained by respiratory gas exchange. For the fourth subject, daytime, nighttime, and daily energy expenditure was calculated by both the two-pair method and decay-curve analysis of urine and saliva samples collected in the morning and at night. Daytime and nighttime energy expenditures differed significantly (p less than 0.05).
通过双标记水（2H2 18O）法测量了四名受试者的能量消耗，以确定是否可以在短时间内测定能量消耗。对三名受试者在实验室环境中进行8小时剧烈运动时进行了研究。在运动前后采集了尿液和血液样本。使用尿液通过双标记水法和两点计算得出，三名受试者在8小时高强度运动（55%最大摄氧量）期间估计消耗的能量为757±118千卡/小时，这与通过呼吸气体交换获得的735±82千卡/小时相比具有优势。对于第四名受试者，通过对早晨和晚上采集的尿液和唾液样本进行双对法和衰变曲线分析来计算白天、夜间和每日的能量消耗。白天和夜间的能量消耗有显著差异（p<0.05）。
