Zhang Jingyun, Shi Jiayuan, Zeng Kehao, Cai Mengjie, Lan Xingguo
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin, China.
Front Plant Sci. 2024 Jan 8;14:1249122. doi: 10.3389/fpls.2023.1249122. eCollection 2023.
, belonging to the cold-specialized lineage Betulaceae, exhibits a unique reproductive strategy where staminate catkins emerge in the first summer and undergo an overwintering process, culminating in flowering in the following year. However, the underlying regulatory mechanism remains unclear. In this study, we investigated the male germline development of in four distinct stages: microsporocytes in Oct. (S1), uninuclear microspores from Dec. (S2) to Mar. of the following year (S3), and bicellular microspores in Apr. (S4). We performed RNA sequencing on mature pollen and the four stages of staminate catkins. Using weighted gene co-expression network analysis (WGCNA), we identified five highly correlated gene modules with distinct expression profiles. These modules exhibited strong correlations with sugar metabolism, cell cycle, flowering, and cell wall dynamics, highlighting their dynamic roles during male germline developmental stages. During the overwintering process, we observed that the expression of transcription factors such as and at the appropriate developmental stages, suggests their significant roles in male germline development. The expression patterns of and suggest their potential involvement in temperature perception during male reproductive development. These findings offer valuable insights into the reproductive success of plants adapting to cold environments.
属于冷适应谱系桦木科，展现出一种独特的繁殖策略，即雄花序在第一个夏天出现并经历越冬过程，最终在次年开花。然而，其潜在的调控机制仍不清楚。在本研究中，我们在四个不同阶段研究了雄配子体发育：10月的小孢子母细胞（S1）、12月至次年3月的单核小孢子（S2至S3）以及4月的二细胞小孢子（S4）。我们对成熟花粉和雄花序的四个阶段进行了RNA测序。使用加权基因共表达网络分析（WGCNA），我们鉴定出五个具有不同表达谱的高度相关基因模块。这些模块与糖代谢、细胞周期、开花和细胞壁动态呈现出强相关性，突出了它们在雄配子体发育阶段的动态作用。在越冬过程中，我们观察到转录因子如[具体转录因子名称1]和[具体转录因子名称2]在适当发育阶段的表达，表明它们在雄配子体发育中具有重要作用。[基因名称1]和[基因名称2]的表达模式表明它们可能参与了雄性生殖发育过程中的温度感知。这些发现为植物适应寒冷环境的繁殖成功提供了有价值的见解。
