Suppr超能文献

采用限定性筛选设计将益生菌包埋在冷冻干燥的海藻酸钙和κ-卡拉胶珠中:全面的特性分析和体外消化研究。

Encapsulation of probiotics in freeze-dried calcium alginate and κ-carrageenan beads using definitive screening design: A comprehensive characterisation and in vitro digestion study.

机构信息

Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.

Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur 721302, West Bengal, India.

出版信息

Int J Biol Macromol. 2024 Feb;258(Pt 2):129279. doi: 10.1016/j.ijbiomac.2024.129279. Epub 2024 Jan 22.

Abstract

This research aimed to evaluate the encapsulation of the probiotic strain, Streptococcus thermophilus, in hydrogels employing sodium alginate (SA) with κ-carrageenan (κC) in gelation baths with varying salt concentrations (CaCl and KCl) followed by freeze-drying. The experimentation was conducted at varying levels of κC (0-0.5 % w/v) and SA (2-4 %). Freeze-dried hydrogels were evaluated based on encapsulation efficiency and loss of viability and further characterised. The study could successfully establish an encapsulation efficiency of 87.814 % and a viability loss of 1.201 log CFU·g for the optimised samples. The SEM micrographs of the optimised Ca-alginate/κC hydrogels exhibited a much denser network with fewer pores. The influence of SA/κC in the beads was confirmed by FTIR and DSC, where distinct peak shifts were observed, which indicated the presence of κC and SA polymers. The probiotic survival under simulated gastrointestinal tract (GIT) conditions, performed in accordance with the INFOGEST protocol, indicated that the optimised Ca-alginate/κC beads had a lower rate of release in the gastric phase and a much higher rate of survival and release in the intestinal phase than the control sample. The swelling behaviour of beads varied due to varying pH in both gastric and intestinal phases, and the κC in the optimised beads affected the swelling ratio significantly.

摘要

本研究旨在评估益生菌菌株嗜热链球菌在水凝胶中的包封,使用海藻酸钠(SA)和角叉菜胶(κC)在不同盐浓度(CaCl 和 KCl)的凝胶浴中进行,并随后进行冷冻干燥。实验在 κC(0-0.5%w/v)和 SA(2-4%)的不同水平上进行。对冷冻干燥水凝胶进行了包封效率和存活率损失的评估,并进行了进一步的特性分析。研究成功建立了优化样品的 87.814%的包封效率和 1.201 对数 CFU·g 的存活率损失。优化的藻酸钙/κC 水凝胶的 SEM 显微照片显示出更密集的网络,孔更少。FTIR 和 DSC 证实了 SA/κC 在珠粒中的存在,观察到明显的峰位移,表明存在 κC 和 SA 聚合物。根据 INFOGEST 协议在模拟胃肠道(GIT)条件下进行的益生菌存活实验表明,优化的藻酸钙/κC 珠粒在胃相中的释放率较低,而在肠相中的存活和释放率较高,优于对照样品。珠粒的溶胀行为因胃相和肠相中的 pH 值不同而有所不同,优化珠粒中的 κC 对溶胀比有显著影响。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验