Guardabasso V, Rodbard D, Munson P J
Am J Physiol. 1987 Mar;252(3 Pt 1):E357-64. doi: 10.1152/ajpendo.1987.252.3.E357.
We have developed a new, general approach to analysis of dose-response curves from bioassay, immunoassay (including radioimmunoassay, immunoradiouretic assay, enzyme-linked immunosorbent assay), and other experimental procedures. It provides a test for parallelism, similarity of shape, and a measure of relative potency for any set of two or more curves. The method uses a constrained smoothing spline function to estimate the curve shape, together with a nonlinear least-squares fitting technique to estimate parameters for relative potency and slope. The use of "constrained splines" permits the analysis of nonlinear dose-response curves that cannot be described by a simple model or equation such as the symmetric four-parameter logistic. A microcomputer program is used for the analysis, providing relative potencies and their SE and evaluation of goodness of fit.
我们已经开发出一种全新的通用方法，用于分析生物测定、免疫测定（包括放射免疫测定、免疫放射尿测定、酶联免疫吸附测定）及其他实验程序中的剂量反应曲线。该方法可对任意两条或更多曲线进行平行性检验、形状相似性检验以及相对效价测定。此方法使用约束平滑样条函数来估计曲线形状，并结合非线性最小二乘法拟合技术来估计相对效价和斜率的参数。“约束样条”的使用使得无法用简单模型或方程（如对称四参数逻辑模型）描述的非线性剂量反应曲线得以分析。分析过程使用微机程序，可提供相对效价及其标准误，并对拟合优度进行评估。
