Vėbraitė Ieva, Bar-Haim Chen, David-Pur Moshe, Hanein Yael
School of Electrical Engineering, Tel Aviv University, Tel Aviv, Israel.
Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, Israel.
Front Neurosci. 2024 Jan 8;17:1288069. doi: 10.3389/fnins.2023.1288069. eCollection 2023.
Electrophysiological investigations of intact neural circuits are challenged by the gentle and complex nature of neural tissues. Bi-directional electrophysiological interfacing with the retina, in its intact form, is particularly demanding and currently there is no feasible approach to achieve such investigations. Here we present a feasibility study of a novel soft multi-electrode array suitable for bi-directional electrophysiological study of the intact retina.
Screen-printed soft electrode arrays were developed and tested. The soft probes were designed to accommodate the curvature of the retina in the eye and offer an opportunity to study the retina in its intact form.
For the first time, we show both electrical recording and stimulation capabilities from the intact retina. In particular, we demonstrate the ability to characterize retina responses to electrical stimulation and reveal stable, direct, and indirect responses compared with conditions.
These results demonstrate the unique performances of the new probe while also suggesting that intact retinas retain better stability and robustness than retinas making them more suitable for characterizing retina responses to electrical stimulation.
完整神经回路的电生理研究因神经组织质地柔软且结构复杂而面临挑战。与完整视网膜进行双向电生理连接的要求尤为苛刻，目前尚无可行方法来开展此类研究。在此，我们展示了一种新型柔软多电极阵列用于完整视网膜双向电生理研究的可行性。
开发并测试了丝网印刷的柔软电极阵列。这些柔软探针旨在适应眼内视网膜的曲率，为以完整形式研究视网膜提供机会。
我们首次展示了从完整视网膜进行电记录和刺激的能力。特别是，我们证明了能够表征视网膜对电刺激的反应，并与对照条件相比揭示稳定、直接和间接反应。
这些结果证明了新型探针的独特性能，同时也表明完整视网膜比分离视网膜具有更好的稳定性和稳健性，使其更适合表征视网膜对电刺激的反应。
