Division of Cardiology, Hongqiao International Institute of Medicine, Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 200336, China.
Shanghai Institute of Pollution Control and Ecological Security, Key Laboratory of Yangtze River Water Environment Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai, China.
Environ Pollut. 2024 Mar 15;345:123441. doi: 10.1016/j.envpol.2024.123441. Epub 2024 Jan 23.
Nanoplastics (NPs) have become common worldwide and attracted increasing attention due to their serious toxic effects. Owing to their higher surface area and volume ratios and ability to easily enter tissues, NPs impose more serious toxic effects than microplastics. However, the effect of NP exposure on vascular stenosis remains unclear. To measure the effects of polystyrene NP (PS-NP) exposure on vascular toxicity, we conducted analyses of blood biochemical parameters, pathological histology, high-throughput sequencing, and bioinformatics. Red fluorescent PS-NPs (100 nm) were effectively uptake by mouse vascular arterial tissue. The uptake of PS-NPs resulted in vascular toxicity, including alterations in lipid metabolism and thickening of the arterial wall. Based on PIWI-interacting RNA (piRNA) sequencing, 1547 and 132 differentially expressed piRNAs (DEpiRNAs) were detected in the PS-NP treatment group after 180 and 30 days, including 787 and 86 upregulated and 760 and 46 downregulated compared with the control group, respectively. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses indicated that the target genes of DEpiRNAs were mostly involved in cell growth and cell motility-related signaling, such as the MAPK signaling pathway. This is the first study to highlight the alteration in piRNA levels in mouse vascular arterial tissue after PS-NP exposure. This study adds to the knowledge regarding the regulatory mechanism of pathological changes induced by PS-NP exposure.
纳米塑料（NPs）在全球范围内变得很普遍，由于其严重的毒性作用而引起了越来越多的关注。由于其具有更高的表面积与体积比，并且容易进入组织，因此 NPs 比微塑料具有更严重的毒性作用。然而，NP 暴露对血管狭窄的影响尚不清楚。为了测量聚苯乙烯纳米颗粒（PS-NP）暴露对血管毒性的影响，我们进行了血液生化参数、病理组织学、高通量测序和生物信息学分析。红色荧光 PS-NP（100nm）被小鼠血管动脉组织有效摄取。PS-NP 的摄取导致了血管毒性，包括脂质代谢的改变和动脉壁的增厚。基于 PIWI 相互作用 RNA（piRNA）测序，在 PS-NP 处理组中分别在 180 天和 30 天后检测到 1547 个和 132 个差异表达的 piRNA（DEpiRNA），与对照组相比，分别有 787 个和 86 个上调，760 个和 46 个下调。基因本体论和京都基因与基因组百科全书富集分析表明，DEpiRNA 的靶基因主要参与细胞生长和细胞运动相关的信号，如 MAPK 信号通路。这是首次研究 PS-NP 暴露后小鼠血管动脉组织中 piRNA 水平的改变。这项研究增加了对 PS-NP 暴露引起的病理变化的调节机制的认识。
