对来自土耳其传统酸奶的菌株进行多位点序列分型及其技术功能作用的表征。
Multilocus sequence typing of and strains from Turkish traditional yoghurts and characterisation of their techno-functional roles.
作者信息
Dikmen Hilal, Goktas Hamza, Demirbas Fatmanur, Kayacan Selma, Ispirli Humeyra, Arici Muhammet, Turker Mustafa, Sagdic Osman, Dertli Enes
机构信息
Food Engineering Department, Chemical and Metallurgical Faculty, Yildiz Technical University, Istanbul, Turkey.
Food Technology Programme, Vocational School, Istinye University, Topkapi Campus, Zeytinburnu, Istanbul, Turkey.
出版信息
Food Sci Biotechnol. 2023 Jul 20;33(3):625-635. doi: 10.1007/s10068-023-01366-2. eCollection 2024 Feb.
UNLABELLED
In this study, and strains from traditional Turkish yoghurts were isolated, identified by 16S rRNA sequencing and genotypically 14 and 6 strains were obtained as distinct strains by MLST analysis. Lactic acid production levels of the strains were higher than strains. HPLC analysis showed that EPS monosaccharide composition of the strains mainly consisted of glucose and galactose. In general, all strains were found to be susceptible for antibiotics, except some strains were resistance to gentamicin and kanamycin. Apart from two strains of , all strains displayed strong auto-aggregation level greater than 95% at 24 h incubation. strains showed higher cell surface hydrophobicity than strains. This study demonstrated the isolation, identification, genotypic discrimination and techno-functional features of wild type yoghurt starter cultures which can potentially find place in industrial applications.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s10068-023-01366-2.
未标记
在本研究中，从传统土耳其酸奶中分离出菌株，通过16S rRNA测序进行鉴定，经多位点序列分型（MLST）分析，分别获得14株和6株不同的菌株。这些菌株的乳酸产生水平高于菌株。高效液相色谱（HPLC）分析表明，这些菌株的胞外多糖单糖组成主要为葡萄糖和半乳糖。总体而言，除部分菌株对庆大霉素和卡那霉素耐药外，所有菌株均对多种抗生素敏感。除两株菌株外，所有菌株在培养24小时时均表现出大于95%的强自聚集水平。菌株的细胞表面疏水性高于菌株。本研究展示了野生型酸奶发酵剂培养物的分离、鉴定、基因分型鉴别及技术功能特性，这些培养物有可能在工业应用中占有一席之地。
补充信息
在线版本包含可在10.1007/s10068-023-01366-2获取的补充材料。
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