Ma Panpan, Guo Guoling, Xu Xiaoqian, Luo Tingyue, Sun Yu, Tang Xiaomei, Heng Wei, Jia Bing, Liu Lun
College of Horticulture, Anhui Agricultural University, Hefei 230036, China.
Plants (Basel). 2024 Jan 20;13(2):309. doi: 10.3390/plants13020309.
Drought and high-temperature stress are the main abiotic stresses that alone or simultaneously affect the yield and quality of pears worldwide. However, studies on the mechanisms of drought or high-temperature resistance in pears remain elusive. Therefore, the molecular responses of , the widely used rootstock in pear production, to drought and high temperatures require further study. Here, drought- or high-temperature-resistant seedlings were selected from many seedlings. The leaf samples collected before and after drought or high-temperature treatment were used to perform RNA sequencing analysis. For drought treatment, a total of 11,731 differentially expressed genes (DEGs) were identified, including 4444 drought-induced genes and 7287 drought-inhibited genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these DEGs were more significantly enriched in plant hormone signal transduction, flavonoid biosynthesis, and glutathione metabolism. For high-temperature treatment, 9639 DEGs were identified, including 5493 significantly upregulated genes and 4146 significantly downregulated genes due to high-temperature stress. KEGG analysis showed that brassinosteroid biosynthesis, arginine metabolism, and proline metabolism were the most enriched pathways for high-temperature response. Meanwhile, the common genes that respond to both drought and high-temperature stress were subsequently identified, with a focus on responsive transcription factors, such as MYB, HSF, bZIP, and WRKY. These results reveal potential genes that function in drought or high-temperature resistance. This study provides a theoretical basis and gene resources for the genetic improvement and molecular breeding of pears.
干旱和高温胁迫是主要的非生物胁迫，单独或同时影响着全球梨树的产量和品质。然而，关于梨树抗旱或抗高温机制的研究仍然不够明确。因此，梨生产中广泛使用的砧木对干旱和高温的分子反应需要进一步研究。在此，从众多砧木幼苗中筛选出抗旱或抗高温的幼苗。采集干旱或高温处理前后的叶片样本进行RNA测序分析。对于干旱处理，共鉴定出11731个差异表达基因（DEG），包括4444个干旱诱导基因和7287个干旱抑制基因。京都基因与基因组百科全书（KEGG）分析表明，这些DEG在植物激素信号转导、类黄酮生物合成和谷胱甘肽代谢中显著富集。对于高温处理，鉴定出9639个DEG，包括5493个因高温胁迫显著上调的基因和4146个显著下调的基因。KEGG分析表明，油菜素类固醇生物合成、精氨酸代谢和脯氨酸代谢是高温响应中最富集的途径。同时，随后鉴定出了对干旱和高温胁迫均有响应的共同基因，重点关注响应转录因子，如MYB、HSF、bZIP和WRKY。这些结果揭示了在抗旱或抗高温中起作用的潜在基因。本研究为梨树的遗传改良和分子育种提供了理论依据和基因资源。
