Department of Pathology, School of Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Department of Obstetrics, Gynecology, and Reproductive Sciences, School of Medicine, University of California, San Diego, La Jolla, CA 92093, USA.
Cell Rep. 2024 Feb 27;43(2):113701. doi: 10.1016/j.celrep.2024.113701. Epub 2024 Jan 26.
Human embryo implantation is remarkably inefficient, and implantation failure remains among the greatest obstacles in treating infertility. Gene expression data from human embryos have accumulated rapidly in recent years; however, identification of the subset of genes that determine successful implantation remains a challenge. We leverage clinical morphologic grading-known for decades to correlate with implantation potential-and transcriptome analyses of matched embryonic and abembryonic samples to identify factors and pathways enriched and depleted in human blastocysts of good and poor morphology. Unexpectedly, we discovered that the greatest difference was in the state of extraembryonic primitive endoderm (PrE) development, with relative deficiencies in poor morphology blastocysts. Our results suggest that implantation success is most strongly influenced by the embryonic compartment and that deficient PrE development is common among embryos with decreased implantation potential. Our study provides a valuable resource for those investigating the markers and mechanisms of human embryo implantation.
人类胚胎着床效率非常低，着床失败仍然是治疗不孕的最大障碍之一。近年来，人类胚胎的基因表达数据迅速积累；然而，确定决定成功着床的基因亚群仍然是一个挑战。我们利用几十年来已知的与着床潜能相关的临床形态学分级，并对匹配的胚胎和无胚胎样本进行转录组分析，以鉴定在形态良好和形态不良的人类囊胚中富集和耗尽的因子和途径。出乎意料的是，我们发现差异最大的是胚胎外原始内胚层（PrE）发育的状态，形态不良的囊胚中相对缺乏 PrE。我们的研究结果表明，着床成功受胚胎腔室的影响最大，而 PrE 发育不足在着床潜能降低的胚胎中很常见。我们的研究为那些研究人类胚胎着床的标记物和机制的人提供了有价值的资源。
