Calcofluor white 在侵袭性真菌病诊断中的价值。

The value of calcofluor white in the diagnosis of invasive fungal diseases.

机构信息

Department of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Ningbo University, Ningbo, Zhejiang, China.

Department of Central Laboratory, The Affiliated Hospital of Medical School of Ningbo University, Ningbo, Zhejiang, China.

出版信息

Diagn Microbiol Infect Dis. 2024 Apr;108(4):116186. doi: 10.1016/j.diagmicrobio.2024.116186. Epub 2024 Jan 14.

DOI:10.1016/j.diagmicrobio.2024.116186

PMID:38278003

Abstract

PURPOSE

To evaluate the value of calcofluor white in the diagnosis of invasive fungal disease (IFD).

METHODS

A total of 84 patients with possible pulmonary fungal infection who underwent bronchoscopy with bronchoalveolar lavage fluid (BALF) were included. All BALF specimens were subjected to Calcofluor white (CFW), potassium hydroxide (KOH) and Gram stains.

RESULTS

CFW has the most sensitivity than KOH and Gram staining. The specificity of CFW was 92.00 %, which was lower than that of Gram staining. The PPVs for CFW, KOH and Gram staining were 94.44 %, 84.62 % and 80.00 % respectively. The NPVs for CFW, KOH and Gram staining was 47.92 %, 32.39 % and 30.38 % respectively. The AUCs of these three methods were 0.748, 0.550 and 0.510 respectively.

CONCLUSION

CFW is superior to KOH and Gram staining in the diagnosis of invasive fungal diseases.

摘要

目的

评估钙荧光白在侵袭性真菌病（IFD）诊断中的价值。

方法

共纳入 84 例经支气管镜肺泡灌洗液（BALF）检查怀疑肺部真菌感染的患者。所有 BALF 标本均进行钙荧光白（CFW）、氢氧化钾（KOH）和革兰染色检查。

结果

CFW 的敏感性高于 KOH 和革兰染色。CFW 的特异性为 92.00%，低于革兰染色。CFW、KOH 和革兰染色的阳性预测值分别为 94.44%、84.62%和 80.00%。CFW、KOH 和革兰染色的阴性预测值分别为 47.92%、32.39%和 30.38%。这三种方法的 AUC 分别为 0.748、0.550 和 0.510。