Chen K Y, Chang Z F
Biochem Biophys Res Commun. 1987 Feb 13;142(3):767-74. doi: 10.1016/0006-291x(87)91480-x.
Possible changes of glycoproteins in IMR-90 human embryonic lung fibroblasts during senescence in vitro were studied by the metabolic labeling technique using radioactive precursors for carbohydrate moieties of glycoproteins. IMR-90 fibroblasts at three different population doubling level (PDL) were incubated with [3H]fucose and [3H]glucosamine for various periods of time. The radioactively labeled glycoproteins were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and fluorography. The results indicated a marked increase, by more than eight-fold on per mg protein basis, of labeling by [3H]fucose in old IMR-90 fibroblasts (PDL = 45) as compared to young (PDL = 22) and middle-age (PDL = 30) IMR-90 fibroblasts. In contrast, no significant difference in [3H]glucosamine labeling was observed in young and old IMR-90 cells.
采用代谢标记技术，利用糖蛋白碳水化合物部分的放射性前体，研究了体外衰老过程中IMR-90人胚肺成纤维细胞中糖蛋白可能发生的变化。将处于三个不同群体倍增水平（PDL）的IMR-90成纤维细胞与[³H]岩藻糖和[³H]葡糖胺孵育不同时间。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和荧光自显影分析放射性标记的糖蛋白。结果表明，与年轻（PDL = 22）和中年（PDL = 30）的IMR-90成纤维细胞相比，衰老的IMR-90成纤维细胞（PDL = 45）中[³H]岩藻糖标记以每毫克蛋白质计显著增加了八倍以上。相比之下，在年轻和衰老的IMR-90细胞中未观察到[³H]葡糖胺标记有显著差异。
