Crabbe J C, Kosobud A, Tam B R, Young E R, Deutsch C M
Alcohol Drug Res. 1987;7(3):163-74.
Using the technique of within-family selective breeding, we have generated mouse lines that differ genetically in sensitivity to the acute hypothermia induced by injection of 3 g/kg ethanol (EtOH). After 5 generations of selection, the difference in maximal hypothermic response between COLD and HOT lines was 1.6 degrees C in the first replicate and 1.2 degrees C in the second replicate. Estimates of realized heritability were h2 = .17 in each replicate. No differences in EtOH metabolism have developed, so the differences between HOT and COLD mice are presumably in neurosensitivity. These lines of animals should be useful for studying the biological mechanisms underlying neurosensitivity to EtOH. In conjunction with other selectively bred lines, they should improve our understanding of the genetic relationships among EtOH neurosensitivity, tolerance and physical dependence.
利用家系内选择性育种技术，我们培育出了对注射3 g/kg乙醇（EtOH）诱导的急性体温过低敏感性存在遗传差异的小鼠品系。经过5代选择，在第一次重复实验中，COLD品系和HOT品系之间最大体温过低反应的差异为1.6摄氏度，在第二次重复实验中为1.2摄氏度。在每个重复实验中，实现遗传力的估计值为h2 = 0.17。乙醇代谢方面未出现差异，因此HOT小鼠和COLD小鼠之间的差异可能在于神经敏感性。这些动物品系应有助于研究对乙醇神经敏感性的生物学机制。与其他选择性培育的品系相结合，它们应能增进我们对乙醇神经敏感性、耐受性和身体依赖性之间遗传关系的理解。
