College of Veterinary Medicine, Pampanga State Agricultural University, Pampanga, the Philippines; College of Arts and Sciences, University of the Philippines Los Baños, Laguna, the Philippines.
Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State University, Ames, IA, USA; Virus and Prion Research Unit, National Animal Disease Center, USDA, Agricultural Research Service, Ames, IA, USA.
Vet Microbiol. 2024 Mar;290:109999. doi: 10.1016/j.vetmic.2024.109999. Epub 2024 Jan 24.
Mycoplasma hyorhinis (Mhr) and M. hyosynoviae (Mhs) are commensal organisms of the upper respiratory tract and tonsils but may also cause arthritis in pigs. In this study, 8-week-old cesarean-derived colostrum-deprived (CDCD) pigs (n = 30; 3 groups, 10 pigs per group, 2 pigs per pen) were inoculated with Mhr, Mhs, or mock-inoculated with culture medium and then pen-based oral fluids were collected at different time points over the 56 days of the experimental study. Oral fluids tested by Mhr and Mhs quantitative real-time PCRs revealed Mhr DNA between day post inoculation (DPI) 5-52 and Mhs DNA between DPI 5-15. Oral fluids were likewise tested for antibody using isotype-specific (IgG, IgA, IgM) indirect ELISAs based on a recombinant chimeric polypeptide of variable lipoproteins (A-G) for Mhr and Tween 20-extracted surface proteins for Mhs. Mhr IgA was detected at DPI 7 and, relative to the control group, significant (p < 0.05) antibody responses were detected in the Mhr group between DPI 12-15 for IgM and DPI 36-56 for both IgA and IgG. In the Mhs group, IgM was detected at DPI 10 and significant (p < 0.05) IgG and IgA responses were detected at DPI 32-56 and DPI 44-56, respectively. This study demonstrated that oral fluid could serve as an effective and convenient antemortem sample for monitoring Mhr and Mhs in swine populations.
猪鼻支原体（Mhr）和滑液支原体（Mhs）是上呼吸道和扁桃体的共生体，但也可能导致猪关节炎。在这项研究中，将 8 周龄剖腹产的初乳剥夺（CDCD）猪（n=30；3 组，每组 10 头猪，每栏 2 头猪）用 Mhr、Mhs 或培养基进行模拟接种，然后在实验研究的 56 天内的不同时间点采集基于栏的口腔液。通过 Mhr 和 Mhs 定量实时 PCR 检测口腔液，发现 Mhr DNA 存在于接种后第 5-52 天，而 Mhs DNA 存在于接种后第 5-15 天。同样，使用针对可变脂蛋白（A-G）嵌合多肽的同种型特异性（IgG、IgA、IgM）间接 ELISA 基于重组嵌合多肽和 Tween 20 提取的表面蛋白检测口腔液中的抗体。在接种后第 7 天检测到 Mhr IgA，与对照组相比，在接种后第 12-15 天检测到 Mhr 组的 IgM 和接种后第 36-56 天的 IgA 和 IgG 抗体均有显著（p<0.05）增加。在 Mhs 组中，在接种后第 10 天检测到 IgM，在接种后第 32-56 天和第 44-56 天检测到显著（p<0.05）的 IgG 和 IgA 反应。本研究表明，口腔液可以作为监测猪群中 Mhr 和 Mhs 的有效和方便的生前样本。
