港海豹（Phoca vitulina）成年血红蛋白α链和β链的氨基酸序列。
Amino-acid sequence of the alpha and beta chains of adult hemoglobin of the harbor seal, Phoca vitulina.
作者信息
Watanabe B, Maita T, Matsuda G, Goodman M, Johnson M L
出版信息
Biol Chem Hoppe Seyler. 1986 Dec;367(12):1251-8. doi: 10.1515/bchm3.1986.367.2.1251.
The complete amino-acid sequences of the alpha and beta chains of adult hemoglobin of harbor seal, Phoca vitulina that belong to carnivora were determined as follows. The alpha and beta chains isolated by chromatography on a CM-cellulose column were digested with trypsin after S-carboxymethylation. Amino-acid sequences of the tryptic peptides derived from both chains were analysed. Comparing the primary structures of the alpha and beta chains of the seal hemoglobin with those of human, dog, bear, badger and cat, 19, 12, 12, 11, and 16 substitutions, respectively, were recognized in the alpha chain, and 12, 10, 4, 6, and 19 (22) in the beta chain.
如下确定了属于食肉目的港海豹（Phoca vitulina）成年血红蛋白α链和β链的完整氨基酸序列。通过在CM - 纤维素柱上进行色谱分离得到的α链和β链，在S - 羧甲基化后用胰蛋白酶消化。分析了两条链衍生的胰蛋白酶肽的氨基酸序列。将海豹血红蛋白的α链和β链的一级结构与人类、狗、熊、獾和猫的进行比较，分别在α链中识别出19、12、12、11和16个取代，在β链中识别出12、10、4、6和19（22）个取代。
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