U.S. Department of Agriculture-Agricultural Research Service, Peanut and Small Grains Research Unit, Stillwater, OK 74075.
U.S. Department of Agriculture-Agricultural Research Service, Hard Winter Wheat Genetics Research Unit, Manhattan, KS 66506.
Phytopathology. 2024 Jun;114(6):1373-1379. doi: 10.1094/PHYTO-09-23-0320-R. Epub 2024 May 28.
Leaf rust, caused by , is a major cause of wheat yield losses globally, and novel leaf rust resistance genes are needed to enhance wheat leaf rust resistance. Teremai Bugdai is a landrace from Uzebekistan that is highly resistant to many races of in the United States. To unravel leaf rust resistance loci in Teremai Bugdai, a recombinant inbred line (RIL) population of Teremai Bugdai × TAM 110 was evaluated for response to race (TNBGJ) and genotyped using single nucleotide polymorphism (SNP) markers generated by genotyping-by-sequencing (GBS). Quantitative trait loci (QTL) analysis using 5,130 high-quality GBS-SNPs revealed three QTLs, , , and , for leaf rust resistance in two experiments. , which is either a new allele or a new resistance locus, was delimited to an ∼19.47-Mb interval between 46.4 and 65.9 Mb on 2DS and explained 31.3 and 33.2% of the phenotypic variance in the two experiments. was mapped in an ∼84.0-kb interval between 719.48 and 719.56 Mb on 6BL, accounting for 33 to 36.8% of the phenotypic variance in two experiments. was placed in a 350-kb interval between 762.41 and 762.76 Mb on 7BL and explained 4.4 to 5.3% of the phenotypic variance. Nine GBS-SNPs flanking these QTLs were converted to kompetitive allele specific PCR (KASP) markers, and these markers can be used to facilitate their introgression into locally adapted wheat lines.
条锈病，由 引起，是全球小麦产量损失的主要原因，需要新的抗条锈病基因来提高小麦的抗条锈病能力。特瑞迈 Bugdai 是一种来自乌兹别克斯坦的地方品种，对美国的许多 菌株具有高度抗性。为了解特瑞迈 Bugdai 中的条锈病抗性基因，对特瑞迈 Bugdai×TAM 110 的重组自交系（RIL）群体进行了对 菌株（TNBGJ）的反应评估，并使用通过测序（GBS）生成的单核苷酸多态性（SNP）标记进行了基因型分析。使用 5130 个高质量 GBS-SNPs 的数量性状基因座（QTL）分析揭示了三个与叶片锈病抗性相关的 QTL， ， ， 和 ，在两个实验中。 ，要么是一个新的 等位基因，要么是一个新的抗性基因座，被限制在 2DS 上的 46.4 到 65.9 Mb 之间的一个大约 19.47-Mb 区间内，解释了两个实验中 31.3%和 33.2%的表型方差。 被定位在 6BL 上的 719.48 到 719.56 Mb 之间的一个大约 84.0-kb 区间内，占两个实验中 33 到 36.8%的表型方差。 位于 7BL 上的 762.41 到 762.76 Mb 之间的一个 350-kb 区间内，解释了 4.4 到 5.3%的表型方差。这三个 QTL 侧翼的 9 个 GBS-SNPs 被转化为竞争性等位基因特异性 PCR（KASP）标记，这些标记可用于促进其导入到当地适应性强的小麦品系中。
