State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, No. 30 South Puzhu Road, Nanjing 211816, People's Republic of China.
Department of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, U.K.
J Agric Food Chem. 2024 Feb 14;72(6):3088-3098. doi: 10.1021/acs.jafc.3c08718. Epub 2024 Jan 28.
Punicic acid is a conjugated linolenic acid with various biological activities including antiobesity, antioxidant, anticancer, and anti-inflammatory effects. It is often used as a nutraceutical, dietary additive, and animal feed. Currently, punicic acid is primarily extracted from pomegranate seed oil, but it is restricted due to the extended growth cycle, climatic limitations, and low recovery level. There have also been reports on the chemical synthesis of punicic acid, but it resulted in a mixture of structurally similar isomers, requiring additional purification/separation steps. In this study, a comprehensive strategy for the production of punicic acid in was implemented by pushing the supply of linoleic acid precursors in a high-oleic oil strain, expressing multiple copies of the fatty acid conjugase gene from , engineering the acyl-editing pathway to improve the phosphatidylcholine pool, and promoting the assembly of punicic acid in the form of triglycerides. The optimal strain with high oil production capacity and a significantly increased punicic acid ratio accumulated 3072.72 mg/L punicic acid, accounting for 6.19% of total fatty acids in fed-batch fermentation, providing a viable, sustainable, and green approach for punicic acid production to substitute plant extraction and chemical synthesis production.
共轭亚麻酸是一种具有多种生物学活性的物质，包括抗肥胖、抗氧化、抗癌和抗炎作用。它常被用作营养保健品、膳食添加剂和动物饲料。目前，主要从石榴籽油中提取共轭亚油酸，但由于生长周期长、气候限制和低回收率，其应用受到限制。也有关于化学合成共轭亚油酸的报道，但得到的是结构相似的异构体混合物，需要进行额外的纯化/分离步骤。在这项研究中，通过在高油酸油菌株中推动亚麻酸前体的供应，表达来自 的多个脂肪酸结合酶基因，工程化酰基编辑途径以提高磷脂酰胆碱库，并促进以三酰基甘油形式组装共轭亚油酸，在 中实施了一种生产共轭亚油酸的综合策略。最佳的高产油菌株和显著增加的共轭亚油酸比例积累了 3072.72 毫克/升共轭亚油酸，占分批补料发酵中总脂肪酸的 6.19%，为替代植物提取和化学合成生产提供了可行、可持续和绿色的共轭亚油酸生产方法。
