Berge R K, Stensland E, Aarsland A, Tsegai G, Osmundsen H, Aarsaether N, Gjellesvik D R
Biochim Biophys Acta. 1987 Mar 13;918(1):60-6. doi: 10.1016/0005-2760(87)90009-9.
Among subcellular fractions of liver homogenates of rats, the clofibroyl-CoA hydrolase activity is found mainly in the cytosolic fraction. It is here shown that the subcellular distribution of clofibroyl-CoA hydrolase appears to be different from the distribution of palmitoyl-CoA hydrolase activity. Thus, in contrast to the case with palmitoyl-CoA, no hydrolysis of clofibroyl-CoA was catalysed by the microsomal fraction. Furthermore, the hydrolysis of palmitoyl-CoA and clofibroyl-CoA in the cytosolic fraction seemed to be catalyzed by two different enzymes. Rats treated with clofibrate (0.3%, w/w) showed a significant increased clofibroyl-CoA hydrolase activity where the cytosolic hydrolase was increased 3.5-fold. Clofibrate administration also elevated the specific clofibroyl-CoA hydrolase activity by factors of 1.7 and 1.5 in the mitochondrial and the light-mitochondrial fractions, respectively. Thus, it is possible that clofibroyl-CoA hydrolase has also a multiorganelle localization.
在大鼠肝脏匀浆的亚细胞组分中，氯贝丁酰辅酶A水解酶活性主要存在于胞质组分中。本文表明，氯贝丁酰辅酶A水解酶的亚细胞分布似乎与棕榈酰辅酶A水解酶活性的分布不同。因此，与棕榈酰辅酶A的情况相反，微粒体组分不能催化氯贝丁酰辅酶A的水解。此外，胞质组分中棕榈酰辅酶A和氯贝丁酰辅酶A的水解似乎由两种不同的酶催化。用氯贝丁酯（0.3%，w/w）处理的大鼠，其氯贝丁酰辅酶A水解酶活性显著增加，其中胞质水解酶增加了3.5倍。给予氯贝丁酯还分别使线粒体和轻线粒体组分中的氯贝丁酰辅酶A水解酶比活性提高了1.7倍和1.5倍。因此，氯贝丁酰辅酶A水解酶也有可能定位于多个细胞器。
