Faculty of Science, Department of Chemistry, Karadeniz Technical University, Trabzon, Turkey.
J Sci Food Agric. 2024 Apr;104(6):3767-3775. doi: 10.1002/jsfa.13261. Epub 2024 Jan 29.
Crataegus orientalis Pall. ex M. Bieb fruit (COPMB) is extensively used as a source of various products in the medicinal-aromatic field and holds the potential for erosion control, ornamental purposes, food source, and economic benefits for forest villagers from its fruits. This study aims to determine the chemical components and biological activities of extracts prepared from COPMB using different solvents.
The present work was designed to define the antioxidant activity [phosphomolybdenum (total antioxidant capacity), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), ferric reducing antioxidant power (FRAP), cupric ion-reducing antioxidant capacity (CUPRAC) and metal chelating activity (MCA)], phytochemical screening analysis, enzyme inhibitor (α-amylase, α-glucosidase and tyrosinase) potential, and liquid chromatography-high resolution mass spectrometry (LC-HRMS) secondary metabolite profiling in different extracts of COPMB. The results of LC-HRMS revealed that fumaric acid was the main phenolic compound in all extracts. Among the extracts, ethyl acetate extract has the highest phytochemical and antioxidant properties [total phenolic content (TPC): 32.5 mg GAE/g, total flavonoid content (TFC): 12.2 mg QE/g, ABTS: 213.0 mg TE/g; CUPRAC: 126.0 mg TE/g, MCA: 145.0 mg EDTA/g; FRAP: 122.8 mg TE/g; TAC: 2.8 mmol TE/g]. Ethyl acetate and methanol extracts are more effective in α-amylase (0.27 ± 0.01 mg/mL; 0.12 ± 0.00 mg/mL), α-glucosidase (0.63 ± 0.02 mg/mL; 0.77 ± 0.02 mg/mL) and tyrosinase (0.03 ± 0.00 mg/mL; 0.03 ± 0.00 mg/mL) enzyme inhibition potentials compared to standard acarbose (0.75 ± 0.02 mg/mL for α-amylase; 1.11 ± 0.03 mg/mL for α-glucosidase) and kojic acid (0.04 ± 0.00 mg/mL).
The findings from this study suggest that COPMB could serve as a valuable source of natural agents for the food and pharmaceutical industry. © 2024 Society of Chemical Industry.
山茱萸（Crataegus orientalis Pall. ex M. Bieb）果实被广泛用作药用和芳香领域各种产品的来源，具有控制侵蚀、观赏、食物来源以及为森林村民带来经济利益的潜力。本研究旨在确定使用不同溶剂从山茱萸果实中提取的提取物的化学成分和生物活性。
本工作旨在定义抗氧化活性[磷钼酸盐（总抗氧化能力）、2,2'- 连氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）、铁还原抗氧化能力（FRAP）、铜离子还原抗氧化能力（CUPRAC）和金属螯合活性（MCA)]、植物化学筛选分析、酶抑制剂（α-淀粉酶、α-葡萄糖苷酶和酪氨酸酶）潜力以及不同山茱萸果实提取物的液相色谱-高分辨率质谱（LC-HRMS）次级代谢产物分析。LC-HRMS 的结果表明，富马酸是所有提取物中的主要酚类化合物。在提取物中，乙酸乙酯提取物具有最高的植物化学和抗氧化特性[总酚含量（TPC）：32.5mg GAE/g，总类黄酮含量（TFC）：12.2mg QE/g，ABTS：213.0mg TE/g；CUPRAC：126.0mg TE/g，MCA：145.0mg EDTA/g；FRAP：122.8mg TE/g；TAC：2.8mmol TE/g]。乙酸乙酯和甲醇提取物对α-淀粉酶（0.27±0.01mg/mL；0.12±0.00mg/mL）、α-葡萄糖苷酶（0.63±0.02mg/mL；0.77±0.02mg/mL）和酪氨酸酶（0.03±0.00mg/mL；0.03±0.00mg/mL）的抑制作用强于标准阿卡波糖（α-淀粉酶 0.75±0.02mg/mL；α-葡萄糖苷酶 1.11±0.03mg/mL）和曲酸（0.04±0.00mg/mL）。
本研究结果表明，山茱萸果实可能是食品和制药工业天然制剂的宝贵来源。© 2024 化学学会。
