利用临时浸没系统在普通篁竹（Bambusa vulgaris Schrad. ex Wendl.）中通过器官发生进行植物的离体繁殖。

In Vitro Propagation of Plants via Organogenesis in Bambusa vulgaris Schrad. ex Wendl Using Temporary Immersion Systems.

机构信息

Instituto de Biotecnología de las Plantas, Universidad Central "Marta Abreu" de Las Villas, Santa Clara, Cuba.

Departamento de Agronomía, Facultad de Ciencias Agropecuarias, Universidad Central "Marta Abreu" de Las Villas, Santa Clara, Cuba.

出版信息

Methods Mol Biol. 2024;2759:183-191. doi: 10.1007/978-1-0716-3654-1_17.

DOI:10.1007/978-1-0716-3654-1_17

PMID:38285150

Abstract

The low multiplication and ex vitro survival rates during acclimatization in the culture house limit the in vitro mass propagation of B. vulgaris. Several scientific studies have described the development of different protocols for bamboo; however, not all of them address the effects of these systems on plant morphology, physiology, and biochemistry in vitro. In this chapter, a complete and optimized protocol is described for plants propagated via organogenesis in temporary immersion systems. In addition, the morphophysiological and biochemical characterization of the plants as well as the survival rates of the obtained plants under ex vitro conditions are analyzed. The obtained results will be the basis for the development of a technology for in vitro propagation as an alternative for the production of plants of the species.

摘要

在培养室进行驯化时，繁殖倍数低和体外存活率低限制了普通麻竹的体外大规模繁殖。有几项科学研究已经描述了不同的毛竹培养方案的发展；然而，并非所有这些方案都涉及这些系统对植物形态、生理和生物化学的影响。在本章中，描述了一种通过组织发生在临时浸没系统中进行植物繁殖的完整和优化的方案。此外，还分析了植物的形态生理和生化特性以及在体外条件下获得的植物的存活率。所获得的结果将为作为该物种植物生产的替代方法的体外繁殖技术的开发提供基础。