Laboratorio Agrícola-Forestal, Centro Nacional de Recursos Genéticos, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP), Tepatitlan de Morelos, Jalisco, Mexico.
Laboratorio de Biotecnología, Campo Experimental Tecomán, Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias (INIFAP), Tecoman, Colima, Mexico.
Methods Mol Biol. 2024;2759:193-198. doi: 10.1007/978-1-0716-3654-1_18.
The Guadalupe cypress (Cupressus guadalupensis S. Watson) is an endangered species included in the list of the NOM-059-SEMARNAT-2010. The presence of wild goats in the habitat has been the greatest threat to the propagation and survival of this species. Therefore, there is a need to generate propagation protocols that facilitate the regeneration of the species. Plant tissue culture offers various possibilities that can facilitate the regeneration of species under some risk. Temporary immersion systems have proven to be an option with various advantages in plant tissue culture, such as increasing the number of seedlings generated and reducing production times, compared to semisolid media. The objective of this chapter is to describe a protocol to propagate Guadalupe cypress tissues in a RITA temporary immersion system.
瓜达卢佩柏（Cupressus guadalupensis S. Watson）是一种濒危物种，被列入 NOM-059-SEMARNAT-2010 名单。在栖息地中，野山羊的存在是该物种繁殖和生存的最大威胁。因此，需要制定繁殖协议，促进该物种的再生。植物组织培养提供了各种可能性，可以在一定程度上促进受威胁物种的再生。与半固态培养基相比，临时浸没系统已被证明是植物组织培养中的一种选择，具有多种优势，例如可以增加产生的幼苗数量并缩短生产时间。本章的目的是描述一种在 RITA 临时浸没系统中繁殖瓜达卢佩柏组织的方案。
