Tobias J W, Bern M M, Netland P A, Zetter B R
Blood. 1987 Apr;69(4):1265-8.
Human monocytes have been shown to penetrate the endothelial layer of large blood vessels and to adhere to the subendothelial basement membrane. To determine the active components of this process, we have studied the ability of monocytes to adhere to isolated components of the subendothelial matrix. Using a quantitative dot-blot adhesion assay, we find that monocytes adhere preferentially to immobilized laminin and elastin. The monocytes adhere less well to fibronectin and bind poorly or not at all to collagen types I and IV, or to heparan sulfate. Monocyte binding to elastin requires an intact, crosslinked molecule as no binding was observed to soluble, acid-alcohol elastin extracts, to pepsin or elastase digests of elastin, to tropoelastin monomer, or to desmosine/isodesmosine crosslinks. Similar binding profiles to elastin, laminin, and fibronectin were seen with the established human leukocyte cell line U937. The promyelocytic cell line HL60 adhered equally well to laminin but showed slightly reduced adhesion to elastin when compared with the fresh monocytes or U937 cells. Freshly isolated human erythrocytes did not demonstrate significant adhesion to fibronectin, laminin, or elastin.
已证明人类单核细胞可穿透大血管的内皮层并黏附于内皮下基底膜。为确定该过程的活性成分，我们研究了单核细胞黏附于内皮下基质分离成分的能力。使用定量斑点印迹黏附试验，我们发现单核细胞优先黏附于固定化的层粘连蛋白和弹性蛋白。单核细胞对纤连蛋白的黏附性较差，对I型和IV型胶原或硫酸乙酰肝素的结合能力较弱或完全不结合。单核细胞与弹性蛋白的结合需要完整的、交联的分子，因为未观察到其与可溶性酸醇弹性蛋白提取物、弹性蛋白酶或胃蛋白酶消化的弹性蛋白、原弹性蛋白单体或锁链素/异锁链素交联物有结合。已建立的人类白细胞细胞系U937对弹性蛋白、层粘连蛋白和纤连蛋白有类似的结合模式。早幼粒细胞系HL60对层粘连蛋白的黏附能力相同，但与新鲜单核细胞或U937细胞相比，对弹性蛋白的黏附略有降低。新鲜分离的人类红细胞对纤连蛋白、层粘连蛋白或弹性蛋白未表现出明显的黏附。
