Wen Shaobai, Yin Xiuran, Zhang Yankun, Diao Xiaoping
Ministry of Education Key Laboratory for Ecology of Tropical Islands, Key Laboratory of Tropical Animal and Plant Ecology of Hainan Province, College of Life Sciences, Hainan Normal University, Haikou, 571158, China; Department of Environmental Sciences, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou, 571199, China.
State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, China.
Mar Environ Res. 2024 Mar;195:106381. doi: 10.1016/j.marenvres.2024.106381. Epub 2024 Jan 26.
Microplastics (MPs) have become a popular research topic due to their potential ramifications on aquatic organisms. To evaluate the ecotoxicological impacts of chronic exposure to different microplastics on marine medaka larvae, we exposed medaka larvae to 200 μg/L of polyethylene (PE-200) and polylactic acid (PLA-200) microplastics for 60 days, respectively. The results indicated that both exposures had no significant effect on fish length/weight and did not result in fish mortality. Notably, the structure of intestinal microbiota was not disrupted either. However, microscopy observations of intestinal tissue suggested that exposure to MPs resulted in inflammation of the intestinal tract of fish and significant atrophy and shedding of small intestinal villus. Linear discriminant analysis Effect Size (LEfSe) showed that intestinal enrichment of Streptomyces occurred in marine medaka larvae in both MPs treatments, while the PE-200 treatment exhibited a significant enrichment. In addition, the PICRUSt2 prediction indicated significant upregulation of the Novobiocin biosynthesis function in gut microbiota in the PE-200 treatment. Overall, multi-level assessment is necessary to determine the risk of exposure of aquatic organisms to MPs.
由于微塑料（MPs）对水生生物可能产生的影响，它已成为一个热门的研究课题。为了评估长期暴露于不同微塑料对海洋青鳉幼鱼的生态毒理学影响，我们分别将青鳉幼鱼暴露于200μg/L的聚乙烯（PE - 200）和聚乳酸（PLA - 200）微塑料中60天。结果表明，两种暴露对鱼的体长/体重均无显著影响，也未导致鱼死亡。值得注意的是，肠道微生物群的结构也未受到破坏。然而，肠道组织的显微镜观察表明，暴露于微塑料会导致鱼的肠道炎症以及小肠绒毛的显著萎缩和脱落。线性判别分析效应大小（LEfSe）表明，在两种微塑料处理的海洋青鳉幼鱼中均出现了链霉菌在肠道中的富集，而PE - 200处理表现出显著富集。此外，PICRUSt2预测表明，在PE - 200处理中，肠道微生物群中的新生霉素生物合成功能显著上调。总体而言，需要进行多层面评估以确定水生生物接触微塑料的风险。
