Shenzhen Public Platform for Screening & Application of Marine Microbial Resources, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, PR China; Institute for Ocean Engineering, Tsinghua University, Beijing 100084, PR China.
State Key Laboratory of Marine Pollution, City University of Hong Kong, Hong Kong 999077, China.
Sci Total Environ. 2021 Mar 10;759:143558. doi: 10.1016/j.scitotenv.2020.143558. Epub 2020 Nov 7.
To assess the potential effects of microplastics (MPs) on gut microbiome, a simple investigation of gut microbial structure is not sufficient, and the function and association of gut microbial structure with host health should also be taken into account. Here, the effects of two particle sizes (2 and 200 μm) of polystyrene MPs (PS-MPs) on the gut microbiota of medaka were evaluated following oral administration at 0.3 and 3.0 μg/mg for 28 days. No change in body length and gut histopathology damage were observed. However, the exposure to PS-MPs significantly decreased fish body weight and disrupted the liver anti-oxidative status. The PS-MPs caused a shift in the gut microbial structure of medaka accompanied by changes in community function, including significant environmental stress, increased carbon degradation/fixation activities, and partially modified nitrogen/phosphorus/sulfur metabolic abilities. Furthermore, the PS-MPs exposure disturbed the glycolipid/tyrosine/energy metabolism and the endocrine balance. A potential correlation between the gut microecology and host response to PS-MPs exposure was also observed. These results indicated that the PS-MPs may contribute to gut-liver axis disruption, which could be the underlying toxicological mechanisms of PS-MPs exposure. This work has improved our knowledge about the relationship between gut microbiota dysbiosis and host metabolic disorders following MPs exposure.
为了评估微塑料（MPs）对肠道微生物组的潜在影响，简单地调查肠道微生物结构是不够的，还应考虑肠道微生物结构的功能及其与宿主健康的关联。在这里，通过口服 28 天，以 0.3 和 3.0μg/mg 的剂量评估了两种粒径（2 和 200μm）聚苯乙烯 MPs（PS-MPs）对斑马鱼肠道微生物群的影响。未观察到体长和肠道组织病理学损伤的变化。然而，PS-MPs 的暴露显著降低了鱼体重量，并破坏了肝脏抗氧化状态。PS-MPs 导致斑马鱼肠道微生物结构发生变化，伴随着群落功能的变化，包括显著的环境应激、增加的碳降解/固定活性以及部分改变的氮/磷/硫代谢能力。此外，PS-MPs 暴露扰乱了糖脂/酪氨酸/能量代谢和内分泌平衡。还观察到 PS-MPs 暴露与肠道微生态和宿主对 PS-MPs 暴露的反应之间存在潜在的相关性。这些结果表明，PS-MPs 可能导致肠道-肝脏轴的破坏，这可能是 PS-MPs 暴露的潜在毒理学机制。这项工作提高了我们对肠道微生物失调与宿主代谢紊乱之间关系的认识，这种关系是在 MPs 暴露后发生的。
