作为抑癌基因在宫颈癌中的作用。
Role of as a Tumor Suppressor in Cervical Cancer.
机构信息
Departamento de Genética y Biología Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), 07360 Ciudad de México, México.
Escuela de Medicina y Ciencias de la Salud, Tecnológico de Monterrey, 14380 México City, México.
出版信息
Front Biosci (Landmark Ed). 2024 Jan 17;29(1):16. doi: 10.31083/j.fbl2901016.
BACKGROUND
The gene is silenced in various types of cancer, including cervical cancer; we recently demonstrated that the gene is also silenced in cervical cancer by hypermethylation of the CpG island in the gene promoter. This study aims to analyze whether could be a tumor suppressor in cervical cancer.
METHODS
After ectopic expressing SLC5A8 in the HeLa cell line, we evaluated its effects on cell behavior both and by Confocal immunofluorescence, cell proliferation, migration assays, and xenograft transplants.
RESULTS
Overexpression of SLC5A8 in the HeLa cell line decreased its proliferation by arresting cancer cells in the G1 phase and inhibiting cellular migration. Furthermore, we observed that pyruvate increased the SLC5A8 effect, inducing S-phase arrest and inhibiting the entry into mitosis. SLC5A8 decreased tumor growth in xenograft transplants, significantly reducing the volume and tumor weight at 35 days of analysis.
CONCLUSIONS
In summary, our results indicate that has a role as a tumor suppressor in cervical cancer.
背景
该基因在包括宫颈癌在内的多种癌症中被沉默；我们最近证明，该基因也通过基因启动子 CpG 岛的高甲基化在宫颈癌中被沉默。本研究旨在分析 是否可以作为宫颈癌的肿瘤抑制因子。
方法
在 HeLa 细胞系中外源性表达 SLC5A8 后，通过共聚焦免疫荧光、细胞增殖、迁移实验和异种移植实验评估其对 和 的影响。
结果
SLC5A8 在 HeLa 细胞系中的过表达通过将癌细胞阻滞在 G1 期并抑制细胞迁移来降低其增殖能力。此外，我们观察到丙酮酸增加了 SLC5A8 的作用，诱导 S 期阻滞并抑制进入有丝分裂。SLC5A8 减少了异种移植中的肿瘤生长，在 35 天的分析时显著降低了体积和肿瘤重量。
结论
综上所述，我们的结果表明 在宫颈癌中具有肿瘤抑制因子的作用。
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