Laboratory of Influenza Research, College of Veterinary Medicine, Chungnam National University, 34134 Daejeon, Republic of Korea.
Institute of Influenza Virus, Chungnam National University, 34134 Daejeon, Republic of Korea.
Front Biosci (Landmark Ed). 2024 Jan 16;29(1):11. doi: 10.31083/j.fbl2901011.
Highly pathogenic H5Nx viruses cause avian influenza, a zoonotic disease that can infect humans. The vaccine can facilitate the prevention of human infections from infected poultry. Our previous study showed that an H5 cleavage-site peptide vaccine containing the polybasic amino acid RRRK could protect chickens from lethal infections of the highly pathogenic H5N6 avian influenza virus.
Chickens immunized with the various polybasic amino combinations (RRRK, RRR, RR, R, RK, and K) of H5 cleavage-site peptides were challenged with highly pathogenic H5N6 avian influenza viruses. The challenged chickens were monitored for survival rate, and viral titers in swabs and tissue samples were measured in Madin-Darby canine kidney (MDCK) cells using the median tissue culture infectious dose 50 (log10 TCID50/mL).
Most H5 cleavage-site vaccines containing various combinations of polybasic amino acids protected chickens from lethal infection. Chickens immunized with the RK-containing peptide combination of the H5 cleavage site were not protected.
The polybasic amino acids (RRRK) of H5 cleavage cleavage-site peptide vaccines are important for protecting chickens against HP H5N6 avian influenza virus. The H5 cleavage cleavage-site peptide containing RK did not protect chickens against the virus.
高致病性 H5Nx 病毒引起禽流感，这是一种可以感染人类的人畜共患病。疫苗可以促进预防人类感染受感染的家禽。我们之前的研究表明，含有多碱性氨基酸 RRRK 的 H5 裂解位点肽疫苗可以保护鸡免受高致病性 H5N6 禽流感病毒的致死性感染。
用含有 H5 裂解位点肽的各种多碱性氨基酸组合（RRRK、RRR、RR、R、RK 和 K）免疫的鸡用高致病性 H5N6 禽流感病毒进行攻毒。监测攻毒后的鸡的存活率，并在 Madin-Darby 犬肾（MDCK）细胞中使用半数组织培养感染剂量 50（log10 TCID50/mL）测量拭子和组织样本中的病毒滴度。
大多数含有各种多碱性氨基酸组合的 H5 裂解位点疫苗可保护鸡免受致死性感染。用 H5 裂解位点含 RK 的肽组合免疫的鸡未得到保护。
H5 裂解位点肽疫苗中的多碱性氨基酸（RRRK）对于保护鸡免受高致病性 H5N6 禽流感病毒至关重要。含有 RK 的 H5 裂解位点肽不能保护鸡免受该病毒的侵害。
