Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou, China.
Pest Manag Sci. 2024 Jun;80(6):2920-2928. doi: 10.1002/ps.8000. Epub 2024 Feb 9.
Pollen is a common plant-derived food source for predatory ladybird beetles under field conditions, yet the potential for pollen to improve the quality of artificial diets remains largely unexplored. In this study, we developed three pollen diets by incorporating varying proportions of canola bee pollen (7.5%, 15.0% and 22.5% with 2.5%, 5.0%, and 7.5% of water, respectively) into a conventional diet. The feeding efficiency of Harmonia axyridis, an omnivorous predator, was evaluated and compared on three pollen diets, a conventional nonpollen diet and pea aphids.
The larvae fed a medium or high pollen diet exhibited significantly higher survival in the 4 instar, pupa and adult stages than those fed a nonpollen diet. These larvae also developed into significantly heavier adults, and their survival rates in adulthood were comparable to those fed pea aphids. Specifically, we revealed the underlying mechanisms through which a high pollen diet enhances pupal development. Consumption of high pollen diet versus nonpollen diet resulted not only in a significant decrease in pupal glycogen content, but also an increase in adult lipid content. Both diet treatments induced similar changes in carbohydrate and glycogen content compared to the aphid diet while exhibiting different alterations in pupal protein content and adult lipid content. Furthermore, the transcriptome analysis revealed that the nutrient metabolism, immune response, and cuticle development pathways were predominantly enriched among the differentially expressed genes (DEGs).
Canola bee pollen offers diverse advantages in terms of rearing H. axyridis larvae with an artificial diet, which will advance the development of effective diets for predaceous coccinellids. © 2024 Society of Chemical Industry.
花粉是野外条件下捕食性瓢虫的常见植物源性食物源，但花粉改善人工饲料质量的潜力在很大程度上仍未得到探索。在这项研究中，我们通过将不同比例的油菜蜂花粉（分别为 7.5%、15.0%和 22.5%，含水量分别为 2.5%、5.0%和 7.5%）掺入常规饮食中，开发了三种花粉饮食。评价了杂食性捕食者异色瓢虫对三种花粉饮食、常规非花粉饮食和豌豆蚜的取食效率，并进行了比较。
中或高花粉饮食喂养的幼虫在 4 龄、蛹和成虫阶段的存活率明显高于非花粉饮食喂养的幼虫。这些幼虫也发育成明显更重的成虫，其成虫期的存活率与喂食豌豆蚜的幼虫相当。具体来说，我们揭示了高花粉饮食促进蛹发育的潜在机制。与非花粉饮食相比，高花粉饮食不仅导致蛹糖原含量显著降低，而且导致成虫脂肪含量增加。与蚜虫饮食相比，这两种饮食处理都导致了类似的碳水化合物和糖原含量的变化，而在蛹蛋白含量和成虫脂质含量方面则表现出不同的变化。此外，转录组分析表明，营养代谢、免疫反应和表皮发育途径在差异表达基因（DEGs）中主要富集。
油菜蜂花粉在饲养异色瓢虫幼虫的人工饮食方面具有多种优势，这将推进捕食性瓢虫有效饮食的开发。 © 2024 化学工业协会。
