Brandt S J, Niedel J E, Bell R M, Young W S
Cell. 1987 Apr 10;49(1):57-63. doi: 10.1016/0092-8674(87)90755-0.
The identification of multiple protein kinase C (PKC) cDNA sequences from rat, bovine, and human tissues has led to the discovery of a family of PKC genes. Using probes complementary to three cDNA sequences from rat brain, designated PKC-I, -II, and -III, the distribution of PKC transcripts in rat tissues was studied by in situ hybridization histochemistry. In brain, PKC-II and PKC-III transcripts colocalized except in the brain stem and spinal cord, where some cells appeared to contain only PKC-III message. PKC-I mRNA had a different, but partially overlapping, pattern of expression. In spleen, PKC-II and PKC-III, but not PKC-I, transcripts were concentrated in the marginal zone of white pulp, the major B-lymphocyte compartment of rat spleen. The distinct distributions of rat PKC mRNAs suggest that different members of the PKC family have specialized functions.
从大鼠、牛和人类组织中鉴定出多个蛋白激酶C（PKC）cDNA序列，从而发现了一个PKC基因家族。使用与大鼠脑的三个cDNA序列互补的探针（分别命名为PKC-I、-II和-III），通过原位杂交组织化学研究了PKC转录本在大鼠组织中的分布。在脑中，PKC-II和PKC-III转录本共定位，但在脑干和脊髓中除外，在这些部位一些细胞似乎只含有PKC-III信息。PKC-I mRNA具有不同但部分重叠的表达模式。在脾脏中，PKC-II和PKC-III转录本（而非PKC-I转录本）集中在白髓的边缘区，这是大鼠脾脏主要的B淋巴细胞区室。大鼠PKC mRNA的不同分布表明PKC家族的不同成员具有特定功能。
