Antimicrobial Resistance of in Children from a Tertiary Pediatric Hospital in Shanghai, China.

BACKGROUND

Our previous study reported a high rate of recurrence in children with () infection (CDI) after conventional antibiotic therapy. Here, we aimed to explore whether metronidazole and vancomycin resistant isolates are circulating in pediatric CDI.

METHODS

Antimicrobial susceptibility testing (AST) using the agar dilution method according to the Clinical and Laboratory Standard Institute (CLSI) were performed on isolates collected from children with CDI between 2019 and 2022 at the Shanghai Children's Hospital. Whole-genome sequencing (WGS) was performed on all isolates, and the presence of antibiotic resistance genes (ARGs) were identified using Resfinder and the Comprehensive Antibiotic Resistance Database (CARD). The presence of plasmid pCD-METRO was detected using SRST2 (v0.2.0) against 8 pCD-METRO coding sequences.

RESULTS

A total of 50 isolates were collected from stools of CDI children. The overall resistance rate on all isolates was 30.00% for metronidazole, 6.00% for vancomycin, 0% for rifaximin, 2.00% for rifampin, 24.00% for meropenem, 100.00% for ceftriaxone and clindamycin, 86.00% for erythromycin, 30.0% for levofloxacin, and 50.0% for tetracycline. Multidrug-resistant (MDR) was presented in 44 isolates (88.00%). Sixteen reported potential ARGs relating with resistance to antibiotic classes of aminoglycoside (), lincosamide-clindamycin-erythromycin (), fluoroquinolones (), glycopeptides (), nucleoside (), tetracycline (), and trimethoprim () were identified. However, the pCD-METRO plasmid and were not detected in any isolates.

CONCLUSION

isolates from children with reduced susceptibility to metronidazole and vancomycin are emerging in pediatric CDI in China. The lack of pCD-METRO plasmid and associated with reduced antibiotic susceptibility suggests there are additional mechanisms of resistance.