Oriowo M A, Bevan J A
Clin Exp Pharmacol Physiol. 1986 Nov-Dec;13(11-12):801-9. doi: 10.1111/j.1440-1681.1986.tb02384.x.
The effect of extracellular calcium ion (Ca2+) depletion, diltiazem, D-600 and TMB-8 on potassium (K+), norepinephrine-(NE) and histamine-induced contractions of the rabbit isolated ovarian artery was studied. K+-induced contractions, which were biphasic, were rapidly lost (congruent to 5 min) when calcium was removed from the physiological saline solution (O-Ca2+ PSS). NE and histamine-induced contractions also declined rapidly under nominal O-Ca2+ conditions without any evidence of a depletion resistant component. However, after 1 h under these conditions there was a partial restoration of their responses. EGTA (10(-3) mol/l) abolished these contractions to NE and histamine but not those in normal PSS. Diltiazem and D-600 were more effective against K+-induced than NE- and histamine-induced contractions while the intracellular Ca2+ antagonist TMB-8 was equally effective against all three responses. Histamine could still evoke contraction of the K+-contracted ovarian artery after this had been completely abolished with diltiazem or D-600 but not TMB-8. These results are interpreted to suggest that K+, NE and histamine probably release intracellular Ca2+ to evoke contractions of the ovarian artery. They differ, however, in the mechanisms they employ to facilitate entry of extracellular calcium, which in turn leads to intracellular calcium release.
研究了细胞外钙离子（Ca2+）缺失、地尔硫䓬、D - 600和TMB - 8对兔离体卵巢动脉钾离子（K+）、去甲肾上腺素（NE）和组胺诱导收缩的影响。K+诱导的收缩呈双相性，当从生理盐溶液（无钙生理盐溶液，O - Ca2+ PSS）中去除钙时，迅速消失（约5分钟）。在名义上无钙条件下，NE和组胺诱导的收缩也迅速下降，没有任何抗耗尽成分的证据。然而，在这些条件下1小时后，它们的反应有部分恢复。乙二醇双四乙酸（EGTA，10(-3) mol/l）消除了对NE和组胺的这些收缩，但对正常生理盐溶液中的收缩没有影响。地尔硫䓬和D - 600对K+诱导的收缩比对NE和组胺诱导的收缩更有效，而细胞内Ca2+拮抗剂TMB - 8对所有三种反应同样有效。在用地尔硫䓬或D - 600完全消除K+收缩的卵巢动脉后，组胺仍能引起收缩，但TMB - 8处理后则不能。这些结果被解释为提示K+、NE和组胺可能通过释放细胞内Ca2+来引起卵巢动脉收缩。然而，它们在促进细胞外钙进入的机制上有所不同，进而导致细胞内钙释放。
