Extracellular Vehicles from Commensal Skin Inhibit Proliferation and Biofilm Formation.

The colonizing microbiota on the body surface play a crucial role in barrier function. () is a significant contributor to skin infection, and the utilization of colonization resistance of skin commensal microorganisms to counteract the invasion of pathogens is a viable approach. However, most studies on colonization resistance have focused on skin bacteria, with limited research on the resistance of skin fungal communities to pathogenic bacteria. Extracellular vehicles (EVs) play an important role in the colonization of microbial niches and the interaction between distinct strains. This paper explores the impact of (), the fungus that dominates the normal healthy skin microbiota, on the proliferation of by examining the distribution disparities between the two microorganisms. Based on the extraction of EVs, the bacterial growth curve, and biofilm formation, it was determined that the EVs of effectively suppressed the growth and biofilm formation of . The presence of diverse metabolites was identified as the primary factor responsible for the growth inhibition of , specifically in relation to glycerol phospholipid metabolism, ABC transport, and arginine synthesis. These findings offer valuable experimental evidence for understanding microbial symbiosis and interactions within healthy skin.