对从豚鼠（Cavia porcellus）几种组织的胞质部分分离得到的苹果酸脱氢酶的比较分析。

Comparative analysis of the malate dehydrogenases isolated from the cytosolic fraction of several tissues of guinea-pig Cavia porcellus.

作者信息

Fornós M, Bozal X, Abante J, Cortés A, Bozal J

出版信息

Comp Biochem Physiol B. 1987;86(1):89-94. doi: 10.1016/0305-0491(87)90179-9.

Abstract

The specific activities of the malate dehydrogenase and lactate dehydrogenase present in the soluble fraction of several guinea-pig tissues are reported. The electrophoretic patterns showed always two forms (A and B) with malate dehydrogenase activity and the five isoenzymes of lactate dehydrogenase. Chromatography of the different soluble fractions through 5' AMP-Sepharose allowed both molecular forms of malate dehydrogenase to be separated and obtained free from lactate dehydrogenase. Comparative studies of the two forms of malate dehydrogenase evidenced that the A and B forms exhibited cytosolic and mitochondrial characteristics, respectively.

摘要

本文报道了几种豚鼠组织可溶性部分中苹果酸脱氢酶和乳酸脱氢酶的比活性。电泳图谱总是显示出具有苹果酸脱氢酶活性的两种形式（A和B）以及乳酸脱氢酶的五种同工酶。通过5'-AMP-琼脂糖对不同的可溶性部分进行层析，可将苹果酸脱氢酶的两种分子形式分离出来，并使其与乳酸脱氢酶分离。对两种形式的苹果酸脱氢酶的比较研究表明，A形式和B形式分别表现出胞质和线粒体的特征。