The School of Medicine, Nankai University, Tianjin, China.
Department of Radiology, Tianjin First Central Hospital, Tianjin Institute of Imaging Medicine, Tianjin, China.
J Magn Reson Imaging. 2024 Nov;60(5):2090-2101. doi: 10.1002/jmri.29265. Epub 2024 Feb 1.
Serum creatinine (Scr) may be not suited to timely and accurately reflect kidney injury related to chronic liver disease. Currently, the ability of arterial spin labeling (ASL) and blood oxygen level-dependent (BOLD) sequences to evaluate renal blood flow (RBF) and blood oxygen in chronic liver disease remains to be verified.
To investigate the value of ASL and BOLD imaging in evaluating hemodynamics and oxygenation changes during kidney injury in an animal model of chronic liver disease.
Prospective.
Chronic liver disease model was established by subcutaneous injection of carbon tetrachloride. Forty-three male Sprague-Dawley rats (8 weeks) were divided into a pathological group (0, 2, 4, 6, 8, 12 weeks, each group: N = 6) and a continuous-scanning group (N = 7).
FIELD STRENGTH/SEQUENCE: 3-T, ASL, BOLD, and T2W.
Regions of interest in the cortex (CO), outer stripe of the outer medulla (OSOM), and inner stripe of the outer medulla (ISOM) are manually delineated. The RBF and T2* values at each time point (0, 2, 4, 6, 8, 12 weeks) are measured and compared. Hematoxylin-eosin score (HE Score, damage area scoring method), alpha-smooth muscle actin (α-SMA), hypoxia-inducible factor-1alpha (HIF-1α), peritubular capillar (PTC) density, Scr, and neutrophil gelatinase-associated lipocalin were harvested.
Analysis of variance, Spearman correlation analysis, Kruskal-Wallis tests, and receiver operating characteristic analysis with the area under the curve (AUC). A P-value <0.05 was considered statistically significant.
Renal RBF and T2* values of CO, OSOM, and ISOM were significantly different from baseline. Both RBF and T2* were significantly correlated with HE Score, α-SMA, HIF-1α, and PTC density (|r| = 0.406-0.853). RBF demonstrated superior diagnostic capability in identifying severe kidney injury in this model of chronic liver disease (AUC = 0.964).
Imaging by ASL and BOLD may detect renal hemodynamics and oxygenation changes related to chronic liver disease early.
5 TECHNICAL EFFICACY: Stage 2.
血清肌酐（Scr）可能不适合及时、准确地反映与慢性肝病相关的肾损伤。目前，动脉自旋标记（ASL）和血氧水平依赖（BOLD）序列评估慢性肝病患者的肾血流（RBF）和血氧的能力仍有待验证。
探讨 ASL 和 BOLD 成像在评估慢性肝病动物模型肾损伤时血流动力学和氧合变化的价值。
前瞻性。
通过皮下注射四氯化碳建立慢性肝病模型。43 只雄性 Sprague-Dawley 大鼠（8 周）分为病理组（0、2、4、6、8、12 周，每组：N=6）和连续扫描组（N=7）。
磁场强度/序列：3T、ASL、BOLD 和 T2W。
手动描绘皮质（CO）、外髓外带（OSOM）和外髓内带（ISOM）的感兴趣区。测量并比较每个时间点（0、2、4、6、8、12 周）的 RBF 和 T2*值。采集苏木精-伊红评分（HE 评分，损伤面积评分法）、α-平滑肌肌动蛋白（α-SMA）、缺氧诱导因子-1α（HIF-1α）、肾小管周毛细血管（PTC）密度、Scr 和中性粒细胞明胶酶相关脂质运载蛋白。
方差分析、Spearman 相关分析、Kruskal-Wallis 检验和受试者工作特征分析，计算曲线下面积（AUC）。P 值<0.05 为有统计学意义。
CO、OSOM 和 ISOM 的肾 RBF 和 T2值与基线相比均有显著差异。RBF 和 T2与 HE 评分、α-SMA、HIF-1α 和 PTC 密度均呈显著相关（|r|=0.406-0.853）。RBF 对诊断慢性肝病模型中严重肾损伤具有较好的诊断能力（AUC=0.964）。
ASL 和 BOLD 成像可以早期检测与慢性肝病相关的肾血流动力学和氧合变化。
5 级 技术效能：2 级。
