Institute for Clinical and Experimental Surgery, Saarland University, Homburg, Germany.
Adv Wound Care (New Rochelle). 2024 Jul;13(7):336-349. doi: 10.1089/wound.2023.0029. Epub 2024 Mar 4.
Chronic wounds represent a considerable burden for the affected patients and the health care system. To overcome this problem, effective treatment strategies are urgently required. In this study, we tested a novel approach by combining platelet-rich plasma (PRP) and microvascular fragments (MVF) to create a prevascularized gel dressing. MVF were enzymatically isolated from the epididymal fat pads of transgenic green fluorescent protein (GFP) C57BL/6J donor mice. Subsequently, 5,000 MVF were suspended in 10 μL murine PRP as carrier and transferred into full-thickness skin wounds within dorsal skinfold chambers of C57BL/6J wild-type mice (PRP+MVF). Wound healing in comparison to empty wounds (control) and wounds filled with PRP alone was repeatedly analyzed throughout 14 days by means of stereomicroscopy, histology, and immunohistochemistry. Planimetric assessment of the wound size over time revealed a significantly accelerated and improved healing of PRP+MVF-treated wounds when compared with PRP-treated and empty control wounds. These wounds also exhibited a significantly higher density of blood and lymph vessels, which originated from the GFP MVF isolates and effectively promoted granulation tissue formation inside the skin defects. This study is the first to combine PRP and MVF for the improvement of wound healing. The combination of PRP and MVF represents a promising approach for the future treatment of wounds that do not heal spontaneously due to poor wound-healing conditions.
慢性创面给患者和医疗系统带来了巨大的负担。为了解决这个问题，迫切需要有效的治疗策略。在这项研究中，我们尝试了一种新的方法，即将富含血小板的血浆（PRP）和微血管片段（MVF）结合起来，制造一种预血管化的凝胶敷料。MVF 是从转绿色荧光蛋白（GFP）C57BL/6J 供体小鼠的附睾脂肪垫中酶解分离得到的。随后，将 5000 个 MVF 悬浮在 10 μL 鼠 PRP 中作为载体，并转移到 C57BL/6J 野生型小鼠背部皮肤囊中的全层皮肤伤口中（PRP+MVF）。通过体视显微镜、组织学和免疫组织化学，在 14 天内反复分析 PRP+MVF 处理的伤口与空伤口（对照）和单独用 PRP 填充的伤口的愈合情况。随着时间的推移，对伤口面积的平面评估显示，与 PRP 处理的和空的对照伤口相比，PRP+MVF 处理的伤口的愈合速度明显加快且得到改善。这些伤口的血液和淋巴管密度也明显更高，这些血管来源于 GFP MVF 分离物，有效地促进了皮肤缺陷内肉芽组织的形成。这项研究首次将 PRP 和 MVF 结合起来，以改善伤口愈合。PRP 和 MVF 的结合为治疗由于愈合条件差而不能自行愈合的伤口提供了一种有前途的方法。
