Institute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China.
University of the Chinese Academy of Sciences, Beijing 100049, China.
Science. 2024 Feb 2;383(6682):eadj9198. doi: 10.1126/science.adj9198.
Mapping single-neuron projections is essential for understanding brain-wide connectivity and diverse functions of the hippocampus (HIP). Here, we reconstructed 10,100 single-neuron projectomes of mouse HIP and classified 43 projectome subtypes with distinct projection patterns. The number of projection targets and axon-tip distribution depended on the soma location along HIP longitudinal and transverse axes. Many projectome subtypes were enriched in specific HIP subdomains defined by spatial transcriptomic profiles. Furthermore, we delineated comprehensive wiring diagrams for HIP neurons projecting exclusively within the HIP formation (HPF) and for those projecting to both intra- and extra-HPF targets. Bihemispheric projecting neurons generally projected to one pair of homologous targets with ipsilateral preference. These organization principles of single-neuron projectomes provide a structural basis for understanding the function of HIP neurons.
绘制单神经元投射图对于理解大脑的整体连接和海马体（HIP）的多种功能至关重要。在这里，我们重建了 10100 个小鼠 HIP 的单神经元投射组，并根据不同的投射模式对 43 种投射组亚型进行了分类。投射目标的数量和轴突末梢的分布取决于 HIP 长轴和短轴上的体细胞位置。许多投射组亚型在特定的 HIP 亚域中富集，这些亚域由空间转录组图谱定义。此外，我们描绘了 HIP 神经元的综合布线图，这些神经元仅投射到 HIP 形成区内（HPF），或者投射到 HPF 内和 HPF 外的目标。双侧投射神经元通常投射到一对具有同侧偏好的同源靶标。这些单神经元投射组的组织原则为理解 HIP 神经元的功能提供了结构基础。
